Journal article
Argonaute slicing is required for heterochromatic silencing and spreading
Science, Vol.313(5790), pp.1134-1137
2006
Abstract
Small interfering RNA (siRNA) guides dimethylation of histone H3 lysine-9 (H3K9me2) via the Argonaute and RNA-dependent RNA polymerase complexes, as well as base-pairing with either RNA or DNA. We show that Argonaute requires the conserved aspartate-aspartate-histidine motif for heterochromatic silencing and for ribonuclease H-like cleavage (slicing) of target messages complementary to siRNA. In the fission yeast Schizosaccharomyces pombe, heterochromatic repeats are transcribed by polymerase II. We show that H3K9me2 spreads into silent reporter genes when they are embedded within these transcripts and that spreading requires read-through transcription, as well as slicing by Argonaute. Thus, siRNA guides histone modification by base-pairing interactions with RNA.
Details
- Title
- Argonaute slicing is required for heterochromatic silencing and spreading
- Authors/Creators
- D.V. Irvine (Author/Creator)M. Zaratiegui (Author/Creator)N.H. Rolia (Author/Creator)D.B. Goto (Author/Creator)D.H. Chitwood (Author/Creator)M.W. Vaughn (Author/Creator)L. Joshua-Tor (Author/Creator)R.A. Martienssen (Author/Creator)
- Publication Details
- Science, Vol.313(5790), pp.1134-1137
- Identifiers
- 991005544746707891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.54 Molecular & Cell Biology - Genetics
- 1.54.100 Epigenetic Regulation
- Web Of Science research areas
- Biochemistry & Molecular Biology
- ESI research areas
- Molecular Biology & Genetics