Journal article
Aromatic metabolism in Rhizobium trifolii-catechol 1,2-dioxygenase
Archives of Microbiology, Vol.141(3), pp.225-228
1985
Abstract
Catechol 1,2-dioxygenase has been purified 46-fold from cells of Rhizobium trifolii TA1 grown on benzoate plus glucose. The dioxygenase had a molecular weight of 107,000 and a sub-unit molecular weight of 59,000. The enzyme had a K m of 2 μM for catechol and also cleaved 4-methylcatechol. The dioxygenase contained 2 g atoms of Fe3+ per mole of enzyme which could be removed by treatment with 1,10-phenanthroline, resulting in a complete loss of activity; reactivation of the enzyme occurred specifically with Fe3+.
Details
- Title
- Aromatic metabolism in Rhizobium trifolii-catechol 1,2-dioxygenase
- Authors/Creators
- Y.P. Chen (Author/Creator) - Murdoch UniversityA.R. Glenn (Author/Creator) - Murdoch UniversityM.J. Dilworth (Author/Creator) - Murdoch University
- Publication Details
- Archives of Microbiology, Vol.141(3), pp.225-228
- Publisher
- Springer Verlag
- Identifiers
- 991005544124107891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.83 Bioengineering
- 3.83.323 Bioremediation
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology