Assessing genetic diversity and performing genome-wide association studies (GWAS) using multiple marker types across desi, kabuli, and wild accessions in chickpea (Cicer arietinum L.)

Pradeep Ruperao; Anil Kumar Vemula; Vinod Kumar Valluri; Sriswathi Manda; Rakesh K Srivastava; Sean Mayes; Rajeev K Varshney; Himabindu Kudapa

doi:10.1002/tpg2.70149

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Assessing genetic diversity and performing genome-wide association studies (GWAS) using multiple marker types across desi, kabuli, and wild accessions in chickpea (Cicer arietinum L.)

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Assessing genetic diversity and performing genome-wide association studies (GWAS) using multiple marker types across desi, kabuli, and wild accessions in chickpea (Cicer arietinum L.)

Pradeep Ruperao, Anil Kumar Vemula, Vinod Kumar Valluri, Sriswathi Manda, Rakesh K Srivastava, Sean Mayes, Rajeev K Varshney and Himabindu Kudapa

The plant genome, Vol.18(4), e70149

2025

DOI: https://doi.org/10.1002/tpg2.70149

PMID: 41276469

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Abstract

Cicer - genetics

Genetic Markers

Genetic Variation

Genome, Plant

Genome-Wide Association Study

Genotype

INDEL Mutation

Plant Breeding

Polymorphism, Single Nucleotide

Genetic diversity is a key aspect of the selection of superior genotypes in crop varietal improvement. Breeding activities in chickpea (Cicer arietinum L.) have successfully enhanced the genetic diversity by introducing variations from wild relatives and landraces. Such diversity was characterized by employing various molecular markers, including single nucleotide polymorphisms (SNPs), insertions and deletions (indels), presence/absence variations (PAVs), and so forth. These marker types through different studies provided different levels of genetic variations from single base to gene structure level. The use of multi-marker types for diversity analysis and genome-wide association studies (GWAS) represents a powerful strategy. In the current study, whole genome re-sequencing data from 593 select chickpea genotypes representing desi, kabuli, and wild types, with over 21 million SNPs, 10 million indels, and 16,117 PAVs, were analyzed. This study demonstrated enhanced diversity in both desi and kabuli types, with wild accessions showing higher diversity compared to landraces. A more comprehensive understanding and broader range of genetic diversity within and between desi, kabuli, and wild accessions, as well as landraces, cultivars, and breeding lines, were captured. The identified novel alleles and gene variations through this analysis offer effective breeding strategies for key traits such as yield, and biotic and abiotic stress tolerance that can significantly contribute to chickpea improvement. Overall, this study highlights the importance of balanced population design, use of multiple marker types in identifying diverse gene pool, and novel marker-trait associations for key/optimal traits paving the way for the development of more resilient chickpea varieties.

Details

Title: Assessing genetic diversity and performing genome-wide association studies (GWAS) using multiple marker types across desi, kabuli, and wild accessions in chickpea (Cicer arietinum L.)
Authors/Creators: Pradeep Ruperao - Global Research Program-Accelerated Crop Improvement, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India
Anil Kumar Vemula - Data Management and Analytical Support, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India
Vinod Kumar Valluri - International Crops Research Institute for the Semi-Arid Tropics
Sriswathi Manda - Global Research Program-Accelerated Crop Improvement, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India
Rakesh K Srivastava - International Crops Research Institute for the Semi-Arid Tropics
Sean Mayes - Global Research Program-Accelerated Crop Improvement, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India
Rajeev K Varshney - Murdoch University, Food Futures Institute
Himabindu Kudapa - International Crops Research Institute for the Semi-Arid Tropics
Publication Details: The plant genome, Vol.18(4), e70149
Publisher: Wiley Periodicals LLC on behalf of Crop Science Society of America.
Number of pages: 19
Grant note: HRD-28/7/2020-HRD-DBT / Department of Biotechnology, Ministry of Science and Technology, India
Identifiers: 991005831949007891
Murdoch Affiliation: Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre; Food Futures Institute
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Genetics & Heredity; Plant Sciences
ESI research areas: Plant & Animal Science