Journal article
Assessing the prospects of Streptomyces sp. RP1A-12 in managing groundnut stem rot disease caused by Sclerotium rolfsii Sacc
Journal of General Plant Pathology, Vol.82(2), pp.96-104
2016
Abstract
Stem rot of groundnut caused by the soilborne pathogen Sclerotium rolfsii can cause significant yield losses. Biological control of stem rot using actinomycetes is a viable alternative to existing fungicidal management. Though actinomycetes are prolific antibiotic producers, reports pertaining to their use in groundnut disease management are limited. Here, actinomycetes were isolated from groundnut rhizospheric soils and screened for antagonism against S. rolfsii through a dual culture assay. Culture filtrates and crude extracts of the potential candidates were screened further for extracellular antifungal activity and characterized for biocontrol and plant-growth-promoting traits. A promising candidate was tested under greenhouse conditions as whole organism as well as crude extracts. Isolate RP1A-12 exhibited high antagonism against S. rolfsii in dual culture assay (69 % inhibition), culture filtrate assay (78–100 % inhibition at various concentrations) and crude extract assay (100 % inhibition with 1 % crude extracts). Moreover, germination of sclerotia of the test pathogen was inhibited with 1 % crude extracts. Strain RP1A-12 produced hydrogen cyanide, lipase, siderophores and indole acetic acid. Oxalic acid production by S. rolfsii was also inhibited by crude extracts of RP1A-12. In greenhouse studies, RP1A-12 reduced stem rot severity. Overall, our results suggest that isolate RP1A-12 has potential biocontrol capabilities against stem rot pathogen. Molecular characterization based on 16S rRNA gene sequencing of RP1A-12 identified it as a species of Streptomyces, closely related to S. flocculus.
Details
- Title
- Assessing the prospects of Streptomyces sp. RP1A-12 in managing groundnut stem rot disease caused by Sclerotium rolfsii Sacc
- Authors/Creators
- S. Jacob (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsR.R. Sajjalaguddam (Author/Creator) - Sreenidhi Institute of Science and TechnologyK.V.K. Kumar (Author/Creator) - International Crops Research Inst. for the Semi-Arid TropicsR. Varshney (Author/Creator) - International Crops Research Inst. for the Semi-Arid TropicsH.K. Sudini (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
- Publication Details
- Journal of General Plant Pathology, Vol.82(2), pp.96-104
- Publisher
- Springer Verlag
- Identifiers
- 991005540163007891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
26 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.556 Microbial Biocontrol
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science