Journal article
Assessment of eosinophils in gastrointestinal inflammatory disease of dogs
Journal of Veterinary Internal Medicine, Vol.32(6), pp.1911-1917
2018
Abstract
Background
Accurate identification of eosinophils in the gastrointestinal (GI) tract of dogs with eosinophilic GI disease (EGID) by histological evaluation is challenging. The currently used hematoxylin and eosin (H&E) staining method detects intact eosinophils but does not detect degranulated eosinophils, thus potentially underrepresenting the number of infiltrating eosinophils.
Objective
To develop a more sensitive method for identifying and quantifying both intact and degranulated eosinophils to diagnose EGID more accurately.
Methods
Endoscopically obtained paraffin‐embedded intestinal biopsy specimens from dogs with GI signs were examined. The study groups were dogs with eosinophilic enteritis (EE), lymphoplasmacytic and mixed enteritis, and control dogs with GI signs but no histologic changes on tissue sections. Consecutive sections were immunolabeled with monoclonal antibodies (mAbs) against the eosinophil granule protein eosinophil peroxidase (Epx) and stained by H&E, respectively. The number of eosinophils was manually quantified and classified as intact or degranulated.
Results
The number of intact eosinophils detected in Epx mAb‐labeled duodenal sections was significantly higher compared with that in H&E‐stained sections, with a similar relationship noted in the colon and stomach. The Epx mAb allowed the unique assessment of eosinophil degranulation. The number of intact and degranulated eosinophils was significantly higher in duodenal lamina propria of the EE and mixed group compared to the control group.
Conclusion
Immunohistochemical detection of Epx provides a more precise method to detect GI tract eosinophils compared to H&E staining and could be used as an alternative and reliable diagnostic tool for assessment of biopsy tissues from dogs with EGID.
Details
- Title
- Assessment of eosinophils in gastrointestinal inflammatory disease of dogs
- Authors/Creators
- I. Bastan (Author/Creator)A.K. Rendahl (Author/Creator)D. Seelig (Author/Creator)M.J. Day (Author/Creator)E.J. Hall (Author/Creator)S.P. Rao (Author/Creator)R.J. Washabau (Author/Creator)P. Sriramarao (Author/Creator)
- Publication Details
- Journal of Veterinary Internal Medicine, Vol.32(6), pp.1911-1917
- Publisher
- American College of Veterinary Internal Medicine
- Identifiers
- 991005542973907891
- Copyright
- © 2018 The Authors.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.65 Allergy
- 1.65.1177 Eosinophilic Disorders
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science