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Association study and expression analysis of stearoyl Co-A desaturase as a candidate gene for fatty acid composition in Indonesian crossbred chickens.pdf
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Journal article
Association study and expression analysis of stearoyl Co-A desaturase as a candidate gene for fatty acid composition in Indonesian crossbred chickens
International Journal of Poultry Science, Vol.17(7), pp.348-355
2018
Abstract
Background and Objective: The stearoyl-CoA desaturase (SCD) gene encodes an enzyme involved in fatty acid (FA) biosynthesis. The aim of the present study was to investigate the association and expression of SCD as a candidate gene for FA composition in Indonesian crossbred chickens.
Materials and Methods: Sixty-two Indonesian crossbred, unsexed chickens (F2 Kampung×broilers) were used. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken.
Results: A single nucleotide polymorphism in coding region c.17492542 C>G of SCD was associated with FA composition, including both unsaturated [linoleic (C18:2n6c) and eicosadienoic (C20:2) acids] and saturated [lauric acid (C12:0)] forms. SCD mRNA expression analysis in liver revealed 6 chickens with extremely high and low FA compositions, of them, high FA birds (n = 3) had higher unsaturated and lower saturated FAs, while the low FA group (n = 3) had lower unsaturated and higher saturated FA levels. SCD expression was higher (p<0.05) in tissues collected from high FA chickens than low FA chickens.
Conclusion:These results will improve the understanding of SCD function in FA composition and will shed light on SCD as a potential candidate in the selection of chickens with higher levels of unsaturated and lower levels of saturated FA.
Details
- Title
- Association study and expression analysis of stearoyl Co-A desaturase as a candidate gene for fatty acid composition in Indonesian crossbred chickens
- Authors/Creators
- A. Gunawan (Author/Creator)E.S. Nurajizah (Author/Creator)K. Listyarini (Author/Creator)A. Furqon (Author/Creator)W. Bilyaro (Author/Creator)C. Sumantri (Author/Creator). Jakaria (Author/Creator)S.H. Akter (Author/Creator)M.J. Uddin (Author/Creator)
- Publication Details
- International Journal of Poultry Science, Vol.17(7), pp.348-355
- Publisher
- Asian Network for Scientific Information (ANSINET)
- Identifiers
- 991005545083307891
- Copyright
- © 2018 Asep Gunawan et al.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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