BAC-derived markers for assaying the stem rust resistance gene, Sr2, in wheat breeding programs

M.D. McNeil; R. Kota; E. Paux; D. Dunn; R. McLean; C. Feuillet; D. Li; X. Kong; E. Lagudah; J.C. Zhang; J.Z. Jia; W. Spielmeyer; M. Bellgard; R. Appels

doi:10.1007/s11032-007-9152-4

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BAC-derived markers for assaying the stem rust resistance gene, Sr2, in wheat breeding programs

Journal article

Peer reviewed

BAC-derived markers for assaying the stem rust resistance gene, Sr2, in wheat breeding programs

M.D. McNeil, R. Kota, E. Paux, D. Dunn, R. McLean, C. Feuillet, D. Li, X. Kong, E. Lagudah, J.C. Zhang, …

Molecular Breeding, Vol.22(1), pp.15-24

2008

DOI: https://doi.org/10.1007/s11032-007-9152-4

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Abstract

Durable broad-spectrum, adult-plant stem rust resistance in wheat conferred by the Sr2 gene has remained effective against Puccinia graminis f. sp tritici worldwide for more than 50 years. The Sr2 gene has been positioned on the physical map of wheat to the distal 25% portion of the short arm of chromosome 3B. Selection for this gene in wheat breeding programs within Australia has been performed so far through the use of the linked pseudo black chaff (PBC) phenotype and of the microsatellite markers Xgwm389 and Xgwm533 that flank the gene. The molecular markers flank a genetic interval of approximately 4 cM equating to a physical distance of over 10 Mbp. Recently, a 3B-specific BAC library was developed and a physical map established for this region. Analysis of the sequence of minimal tiling path-BAC clones within the region containing the Sr2 gene enabled the development of three new markers that were mapped within the Xgwm389–Xgwm533 genetic interval and tightly linked to the Sr2 gene. Screening a wide range of germplasm containing the Sr2 gene with these markers demonstrated their usefulness for marker-assisted selection in Australian wheat breeding programs.

Details

Title: BAC-derived markers for assaying the stem rust resistance gene, Sr2, in wheat breeding programs
Authors/Creators: M.D. McNeil (Author/Creator) - The University of Queensland
R. Kota (Author/Creator) - Canberra, Australia
E. Paux (Author/Creator) - Génétique, Diversité, Écophysiologie des Céréales
D. Dunn (Author/Creator) - Murdoch University
R. McLean (Author/Creator) - Agriculture and Food
C. Feuillet (Author/Creator) - Génétique, Diversité, Écophysiologie des Céréales
D. Li (Author/Creator) - Murdoch University
X. Kong (Author/Creator) - Civil Aviation Authority
E. Lagudah (Author/Creator) - Canberra, Australia
J.C. Zhang (Author/Creator) - Civil Aviation Authority
J.Z. Jia (Author/Creator) - Civil Aviation Authority
W. Spielmeyer (Author/Creator) - Canberra, Australia
M. Bellgard (Author/Creator) - Murdoch University
R. Appels (Author/Creator) - Murdoch University
Publication Details: Molecular Breeding, Vol.22(1), pp.15-24
Publisher: Kluwer Academic Publishers
Identifiers: 991005542611807891
Copyright: 2008 Springer Science + Business Media B.V.
Murdoch Affiliation: Centre for Comparative Genomics; State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Agronomy; Genetics & Heredity; Horticulture; Plant Sciences
ESI research areas: Plant & Animal Science