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BSA‑seq and genetic mapping identified candidate genes for branching habit in peanut
Journal article   Peer reviewed

BSA‑seq and genetic mapping identified candidate genes for branching habit in peanut

J. Pan, X. Zhou, N. Ahmad, K. Zhang, R. Tang, H. Zhao, J. Jiang, M. Tian, C. Li, A. Li, …
Theoretical and Applied Genetics
2022
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Abstract

Lateral branch angel (LBA) is an important plant architecture trait of peanut, which plays key role in lodging, peg soil penetration and pod yield. However, there are few reports of fine mapping and quantitative trait loci (QTLs)/cloned genes for LBA in peanut. In this project, a mapping population was constructed using a spreading variety Tifrunner and the erect variety Fuhuasheng. Through bulked segregant analysis sequencing (BSA-seq), a major gene related to LBA, named as AhLBA1, was preliminarily mapped at the region of Chr.15: 150-160 Mb. Then, using traditional QTL approach, AhLBA1 was narrowed to a 1.12 cM region, corresponding to a 136.65-kb physical interval of the reference genome. Of the nine genes housed in this region, three of them were involved in hormone metabolism and regulation, including one “F-box protein” and two “2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase (2OG oxygenase)” encoding genes. In addition, we found that the level of some classes of cytokinin (CK), auxin and ethylene showed significant differences between spreading and erect peanuts at the junction of main stem and lateral branch. These findings will aid further elucidation of the genetic mechanism of LBA in peanut and facilitating marker-assisted selection (MAS) in the future breeding program.

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Collaboration types
Domestic collaboration
International collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.4 Crop Science
3.4.159 Arabidopsis
Web Of Science research areas
Agronomy
Genetics & Heredity
Horticulture
Plant Sciences
ESI research areas
Agricultural Sciences
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