Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice

K.A. Martinez; J. Romano-Keeler; J.P. Zackular; D.J. Moore; R.M. Brucker; C. Hooper; S. Meng; N. Brown; S. Mallal; J. Reese; D.M. Aronoff; H. Shin; M.G. Dominguez-Bello; J-H Weitkamp

doi:10.1371/journal.pone.0197439

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Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice

Journal article

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Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice

K.A. Martinez, J. Romano-Keeler, J.P. Zackular, D.J. Moore, R.M. Brucker, C. Hooper, S. Meng, N. Brown, S. Mallal, J. Reese, …

PLoS ONE, Vol.13(5)

2018

DOI: https://doi.org/10.1371/journal.pone.0197439

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Abstract

Bacterial DNA has been reported in the placenta and amniotic fluid by several independent groups of investigators. However, it's taxonomic overlap with fetal and maternal bacterial DNA in different sites has been poorly characterized. Here, we determined the presence of bacterial DNA in the intestines and placentas of fetal mice at gestational day 17 (n = 13). These were compared to newborn intestines (n = 15), maternal sites (mouth, n = 6; vagina, n = 6; colon, n = 7; feces, n = 8), and negative controls to rule out contamination. The V4 region of the bacterial 16S rRNA gene indicated a pattern of bacterial DNA in fetal intestine similar to placenta but with higher phylogenetic diversity than placenta or newborn intestine. Firmicutes were the most frequently assignable phylum. SourceTracker analysis suggested the placenta as the most commonly identifiable origin for fetal bacterial DNA, but also over 75% of fetal gut genera overlapped with maternal oral and vaginal taxa but not with maternal or newborn feces. These data provide evidence for the presence of bacterial DNA in the mouse fetus.

Details

Title: Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice
Authors/Creators: K.A. Martinez (Author/Creator) - New York University
J. Romano-Keeler (Author/Creator) - Vanderbilt University Medical Center
J.P. Zackular (Author/Creator) - Vanderbilt University Medical Center
D.J. Moore (Author/Creator) - Vanderbilt University Medical Center
R.M. Brucker (Author/Creator) - Vanderbilt University
C. Hooper (Author/Creator) - Vanderbilt University Medical Center
S. Meng (Author/Creator) - Vanderbilt University Medical Center
N. Brown (Author/Creator) - Vanderbilt University Medical Center
S. Mallal (Author/Creator) - Vanderbilt University Medical Center
J. Reese (Author/Creator) - Vanderbilt University
D.M. Aronoff (Author/Creator) - Vanderbilt University Medical Center
H. Shin (Author/Creator) - New York University
M.G. Dominguez-Bello (Author/Creator) - Department of Microbiology, Sackler Institute, New York, New York, United States of America.
J-H Weitkamp (Author/Creator) - Vanderbilt University Medical Center
Publication Details: PLoS ONE, Vol.13(5)
Publisher: Public Library of Science
Identifiers: 991005542721107891
Copyright: © 2018 Martinez et al
Murdoch Affiliation: Institute for Immunology and Infectious Diseases
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.120 Inflammatory Bowel Diseases & Infections; 1.120.384 Gut Microbiota
Web Of Science research areas: Microbiology
ESI research areas: Multidisciplinary