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Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma
Journal article   Open access   Peer reviewed

Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma

Julien Wist, Philipp Nitschke, Ricardo Conde, Angela de Diego, Maider Bizkarguenaga, Samantha Lodge, Drew Hall, Zhonglin Chai, Wenjun Wang, Sudhir Kowlessur, …
Analytical chemistry (Washington), Vol.97(12), pp.6399-6409
2025
PMID: 40095953
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CC BY-NC-ND V4.0 Open Access

Abstract

We report the successful development and translation of high-field nuclear magnetic resonance (NMR) based comprehensive lipoprotein analysis to routine benchtop systems. This demonstrates the potential to reimagine population level cardiovascular disease risk analysis and individual level screening based on blood sampling. Using a quantitative calibration approach, we obtained stable and reproducible results from multiple sites, despite reduced spectral dispersion and sensitivity at lower field strengths. Our study shows that 25 out of 28 major lipoprotein parameters, including key cardiometabolic risk markers, were faithfully measured using benchtop NMR systems within 15 min. This development has significant implications for making a powerful diagnostic tool widely available, enhancing the potential for longitudinal personalized medicine through molecular phenotyping in the clinic.

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Source: InCites

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Collaboration types
Domestic collaboration
International collaboration
Citation topics
2 Chemistry
2.211 Mass Spectrometry
2.211.990 Metabolomics
Web Of Science research areas
Chemistry, Analytical
ESI research areas
Chemistry
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