Biochemical Defense Arsenal, Genes/QTLs and Transcripts for Imparting Anthracnose Resistance in Common bean (Phaseolus vulgaris L.)

Safoora Shafi; Mohd Tahir; Vanya Bawa; Farkhandah Jan; Neeraj Choudhary; Mohd Anwar Khan; Yogita Singh; Upendra Kumar; Bilal Ahmad Bhat; Waseem Ahmad Dar; Parvaze Ahmad Sofi; Mohd. Ashraf Bhat; Bilal Ahmad Padder; Asif Bashir Shikari; Rajneesh Paliwal; Rajeev Kumar Varshney; Reyazul Rouf Mir

doi:10.1016/j.stress.2024.100609

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Biochemical Defense Arsenal, Genes/QTLs and Transcripts for Imparting Anthracnose Resistance in Common bean (Phaseolus vulgaris L.)

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Biochemical Defense Arsenal, Genes/QTLs and Transcripts for Imparting Anthracnose Resistance in Common bean (Phaseolus vulgaris L.)

Safoora Shafi, Mohd Tahir, Vanya Bawa, Farkhandah Jan, Neeraj Choudhary, Mohd Anwar Khan, Yogita Singh, Upendra Kumar, Bilal Ahmad Bhat, Waseem Ahmad Dar, …

Plant stress (Amsterdam), Vol.14, 100609

2024

DOI: https://doi.org/10.1016/j.stress.2024.100609

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Abstract

Anthracnose

Candidate genes

Colletotrichum lindemuthianum

Common bean

GWAS

qRT‒PCR

Transcriptomics

Anthracnose (ANT), caused by Colletotrichum lindemuthianum, is the most devastating disease affecting common bean (Phaseolus vulgaris L.), leading to significant yield losses in the Western Himalayas. The study provides a comprehensive understanding of ANT resistance via trait phenotyping, biochemical profiling, genome-wide association studies (GWASs), and RNA sequencing. The assessment of bean association mapping panel in different environments revealed a diverse spectrum of resistance levels. Biochemical analysis revealed distinctive defense responses against ANT infection among different genotypes. GWAS approach identified 24 significant marker‒trait associations (MTAs) distributed across all 11 bean chromosomes. Notably, 03 MTAs (BMr205, BMr269 and BMr244) present on chromosome Pv07 were validated for ANT, and the remaining MTAs were novel MTAs for ANT. Transcriptome sequencing of resistant (PBG-3) and susceptible (PBG-26) genotypes under mock and 120-hour post inoculation conditions revealed key differentially expressed genes, such as leucine-rich repeat domain-containing protein (PHAVU_007G087700g), NB-ARC domain-containing protein (PHAVU_003G002500g) and transcription factors pivotal for disease resistance. The expression patterns of four genes (PHAVU_007G087700g, PHAVU_003G002500g, PHAVU_007G056100g and PHAVU_003G003000g) were validated through quantitative reverse transcription polymerase chain reaction (qRT‒PCR). Furthermore, the integration of GWAS-identified candidate genes with transcriptomics and cross-referencing with previous studies validated overlapping regions and common candidate genes, enriching our understanding of the genetic basis of ANT resistance. Therefore, the results offer a holistic perspective on ANT resistance in common bean, providing a foundation for targeted breeding efforts. The identified potential candidate genes and associated pathways will contribute valuable insights into the development of ANT resistant common bean varieties.

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Title: Biochemical Defense Arsenal, Genes/QTLs and Transcripts for Imparting Anthracnose Resistance in Common bean (Phaseolus vulgaris L.)
Authors/Creators: Safoora Shafi - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Mohd Tahir - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Vanya Bawa - Plant (United States)
Farkhandah Jan - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Neeraj Choudhary - Plant (United States)
Mohd Anwar Khan - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Yogita Singh - University of Massachusetts Amherst
Upendra Kumar - M.J.P. Rohilkhand University
Bilal Ahmad Bhat - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Waseem Ahmad Dar - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Parvaze Ahmad Sofi - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Mohd. Ashraf Bhat - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Bilal Ahmad Padder - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Asif Bashir Shikari - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Rajneesh Paliwal - The International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, Nigeria
Rajeev Kumar Varshney - Murdoch University, Food Futures Institute
Reyazul Rouf Mir - Division of Genetics & Plant Breeding, Faculty of Agriculture (FoA), SKUAST-Kashmir, Wadura Campus, Sopore-193201, Kashmir, J&K, India
Publication Details: Plant stress (Amsterdam), Vol.14, 100609
Publisher: Elsevier B.V
Identifiers: 991005707256507891
Murdoch Affiliation: Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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