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Biocompatibility of synthesised nano-porous anodic Aluminium oxide membranes for use as a cell culture substrate for Madin-Darby Canine Kidneys cells: A preliminary study
Journal article   Open access   Peer reviewed

Biocompatibility of synthesised nano-porous anodic Aluminium oxide membranes for use as a cell culture substrate for Madin-Darby Canine Kidneys cells: A preliminary study

G.E.J. Poinern, N. Ali, C. Berry, P. Singh, S. Berchmans and D. Fawcett
Journal of Tissue Science & Engineering, Vol.3(3)
2012
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Abstract

In this study we investigate for the first time the biomedical potential of using a membrane made from anodic aluminium oxide (AAO) for culturing the Madin-Darby Canine Kidney (MDCK) epithelial cell line. Nano-porous aluminium oxide membranes exhibit interesting properties such as high porosity, which allows the exchange of molecules and nutrients across the membrane and can be made with highly specific pore sizes that can be preselected by adjusting the controlling parameters of a temperature controlled two-step anodization process. The cellular response and interactions of the MDCK cell line with the synthesised nano-porous AAO membrane, a commercially available membrane and a glass control were assessed by investigating cell adhesion, morphology and proliferation.

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