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Bioinformatics and microbial biodiversity: Analysis of vibrios by the GenEnv system
Journal article   Peer reviewed

Bioinformatics and microbial biodiversity: Analysis of vibrios by the GenEnv system

A. Paparini, D. Santoni and V. Romano Spica
Journal of Preventive Medicine and Hygiene, Vol.47(3), pp.100-104
2006
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Abstract

Sequence-based approaches to prokaryotic systematics and typing represent a modern and promising strategy in epidemiology and environmental microbiology. GenEnv, a database-driven system for bacterial typing, was developed in order to provide user friendly tools for supporting biomolecular analysis of bacteria. The family Vibrionaceae represents a heterogeneous taxon of aquatic microrganisms, harbouring a plethora of genomes currently analyzed by different molecular techniques. Under the query "Vibrio", GenEnv retrieved 256 organisms, included in a total number of 19 families. Overall, 548 sequences, comprising 16S rRNA (n = 402), rpoB (n = 1), gyrB (n = 145) were available. In addition, GenEnv system allowed primer design, homology analysis and restriction maps, for immediate applications to the study of Vibrionaceae.

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