Journal article
Bovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment
Proteomics, Vol.15(11), pp.1906-1920
2015
Abstract
A significant proportion of mammalian fertilization is mediated through the proteomic composition of the sperm surface. These protein constituents can present as biomarkers to control and regulate breeding of agricultural animals. Previous studies have addressed the bovine sperm cell apical plasma membrane (PM) proteome with nitrogen cavitation enrichment. Alternative workflows would enable to expand the compositional data more globally around the entire sperm's surface. We used a cell surface biotin‐labeling in combination with differential centrifugation to enrich sperm surface proteins. Using nano‐LC MS/MS, 338 proteins were confidently identified in the PM‐enriched proteome. Functional categories of sperm–egg interaction, protein turnover, metabolism as well as molecular transport, spermatogenesis, and signal transduction were represented by proteins with high quantitative signal in our study. A highly significant degree of enrichment was found for transmembrane and PM‐targeted proteins. Among them, we also report proteins previously not described on bovine sperm (CPQ, CD58, CKLF, CPVL, GLB1L3, and LPCAT2B) of which CPQ and CPVL cell surface localization was further validated. A descriptive overview of the bovine sperm PM integral and peripheral proteins is provided to complement future studies on animal reproduction and its relation to sperm cell surface. All MS data have been deposited in the ProteomeXchange with identifier PXD001096 (http://proteomecentral.proteomexchange.org/dataset/PXD001096).
Details
- Title
- Bovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment
- Authors/Creators
- S. Kasvandik (Author/Creator)G. Sillaste (Author/Creator)A. Velthut-Meikas (Author/Creator)A-V Mikelsaar (Author/Creator)T. Hallap (Author/Creator)P. Padrik (Author/Creator)T. Tenson (Author/Creator)Ü. Jaakma (Author/Creator)S. Kõks (Author/Creator)A. Salumets (Author/Creator)
- Publication Details
- Proteomics, Vol.15(11), pp.1906-1920
- Publisher
- John Wiley & Sons Ltd.
- Identifiers
- 991005540420007891
- Copyright
- © 2015 WILEY‐VCH Verlag GmbH & Co. KGaA
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
69 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.81 Reproductive Biology
- 1.81.176 Male Fertility
- Web Of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- ESI research areas
- Biology & Biochemistry