Journal article
CD1 genotyping of patients with Mycobacterium malmoense pulmonary disease
Tissue Antigens, Vol.58(1), pp.19-23
2001
Abstract
Mycobacterium malmoense is an opportunistic mycobacterium that occasionally causes disease in non-immunosuppressed individuals. As only a few individuals exposed to these organisms actually develop clinical disease, it is possible there is a genetic component to susceptibility. CD1 molecules are capable of presenting antigens from more virulent mycobacteria to T cells; therefore, we were interested in discovering whether recently described polymorphisms in CD1 molecules modulated susceptibility to M. malmoense pulmonary disease. The CD1 system comprises five genes (CD1A, -B, -C, -D, and -E) located on chromosome 1 (1q22–23). CD1 molecules are structurally and functionally related to major histocompatibility complex (MHC) class I molecules and are expressed on dedicated antigen-presenting cells. The primary function of CD1 molecules is to present lipid and glycolipid antigens to T cells. We have developed an allele-specific polymerase chain reaction-sequence-specific primer (PCR-SSP) method of CD1 genotyping. Using this method, we compared the allele and haplotype frequencies of CD1 in 49 HIV-negative patients with M. malmoense pulmonary disease with those in 342 normal controls. The CD1A and CD1E alleles were nominally identified as CD1A*01, CD1A*02, CD1E*01 and CD1E*02, and the control gene frequencies were found to be 5%, 95%, 67% and 33%, respectively. No significant difference was observed between the patient and control cohorts. Positive linkage disequilibrium values of 0.73 were observed between CD1A*02 and CD1E*01 (P<0.0001; χ2 test), and 0.94 between CD1A*01 and CD1E*02 (P<0.0001; χ2 test). Typing was also performed for two previously described CD1D alleles (CD1D*01 and CD1D*02), although only CD1D*01 was detected.
Details
- Title
- CD1 genotyping of patients with Mycobacterium malmoense pulmonary disease
- Authors/Creators
- D.C. Jones (Author/Creator) - Churchill HospitalC.M. Gelder (Author/Creator) - University of WalesT. Ahmad (Author/Creator) - University of OxfordI.A Campbell (Author/Creator) - University Hospital LlandoughM.C.N.M. Barnardo (Author/Creator)K.I. Welsh (Author/Creator) - Churchill HospitalS.E. Marshall (Author/Creator) - Churchill HospitalM. Bunce (Author/Creator) - Churchill Hospital
- Publication Details
- Tissue Antigens, Vol.58(1), pp.19-23
- Publisher
- John Wiley and Sons
- Identifiers
- 991005541927507891
- Copyright
- 2001 Munksgaard
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.1021 Natural Killer Cells
- Web Of Science research areas
- Cell Biology
- Immunology
- Pathology
- ESI research areas
- Molecular Biology & Genetics