Journal article
CD14-positive hepatic monocytes/macrophages increase in hereditary hemochromatosis
Liver International, Vol.24(5), pp.446-451
2004
Abstract
Background/Aims: Iron overload in hereditary hemochromatosis (HH) may result in hepatic fibrosis and cirrhosis, primarily due to collagen production by hepatic stellate cells that become activated to myofibroblasts. Endotoxin-responsive monocytes/macrophages (CD14-positive) are potential sources of profibrogenic factors. The aims of this study were to determine (1) whether CD14-positive monocytes/macrophages are present in the livers of patients with HH and (2) the potential relationship between CD14-positive cells and hepatic fibrosis in HH.
Methods: HH was diagnosed using standard clinical, biochemical and genotypic parameters. Liver specimens from HH patients and control subjects were immunostained for CD14, CD68 and α-smooth muscle actin (α-SMA) and the number of cells expressing these antigens was determined. Fibrosis was assessed by routine histological methods.
Results: The total number of hepatic CD68-positive monocytes/macrophages was similar in HH patients and control subjects; however, there was a nine-fold increase in the number of CD14-positive monocytes/macrophages in HH patients. Control subjects had very low levels of hepatic CD14 expression. In HH livers with advanced fibrosis, CD14-positive monocytes/macrophages were often associated with fibrous septa containing myofibroblasts expressing α-SMA.
Conclusions: There was a substantial increase in hepatic CD14-positive monocytes/macrophages in HH and, in livers with advanced fibrosis, these cells were often associated with fibrous septa and septal myofibroblasts. The total number of monocytes/macrophages was similar in HH and control livers. In control human liver, Kupffer cells had a very low expression of CD14. These findings suggest that CD14-positive monocytes/macrophages may contribute to the process of hepatic fibrogenesis in HH.
Details
- Title
- CD14-positive hepatic monocytes/macrophages increase in hereditary hemochromatosis
- Authors/Creators
- K.L. Leicester (Author/Creator) - Saint Louis UniversityJ.K. Olynyk (Author/Creator) - Fremantle HospitalE.M. Brunt (Author/Creator) - Saint Louis UniversityR.S. Britton (Author/Creator) - Saint Louis UniversityB.R. Bacon (Author/Creator) - Saint Louis University
- Publication Details
- Liver International, Vol.24(5), pp.446-451
- Publisher
- Wiley-Blackwell
- Identifiers
- 991005540106707891
- Copyright
- 2004 Blackwell Munksgaard
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.184 Physiology & Metals
- 1.184.573 Iron Metabolism
- Web Of Science research areas
- Gastroenterology & Hepatology
- ESI research areas
- Clinical Medicine