CNI1/ATL31, a RING-type ubiquitin ligase that functions in the carbon/nitrogen response for growth phase transition in Arabidopsis seedlings

T. Sato; S. Maekawa; S. Yasuda; Y. Sonoda; E. Katoh; T. Ichikawa; M. Nakazawa; M. Seki; K. Shinozaki; M. Matsui; D.B. Goto; A. Ikeda; J. Yamaguchi

doi:10.1111/j.1365-313X.2009.04006.x

Back

CNI1/ATL31, a RING-type ubiquitin ligase that functions in the carbon/nitrogen response for growth phase transition in Arabidopsis seedlings

Journal article

Peer reviewed

CNI1/ATL31, a RING-type ubiquitin ligase that functions in the carbon/nitrogen response for growth phase transition in Arabidopsis seedlings

T. Sato, S. Maekawa, S. Yasuda, Y. Sonoda, E. Katoh, T. Ichikawa, M. Nakazawa, M. Seki, K. Shinozaki, M. Matsui, …

The Plant Journal, Vol.60(5), pp.852-864

2009

DOI: https://doi.org/10.1111/j.1365-313X.2009.04006.x

Files and links (1)

url

Free to Read *No subscription requiredView

Abstract

Plants are able to sense and respond to changes in the balance between carbon (C) and nitrogen (N) metabolite availability, known as the C/N response. During the transition to photoautotrophic growth following germination, growth of seedlings is arrested if a high external C/N ratio is detected. To clarify the mechanisms for C/N sensing and signaling during this transition period, we screened a large collection of FOX transgenic plants, overexpressing full-length cDNAs, for individuals able to continue post-germinative growth under severe C/N stress. One line, cni1-D (carbon/nitrogen insensitive 1-dominant), was shown to have a suppressed sensitivity to C/N conditions at both the physiological and molecular level. The CNI1 cDNA encoded a predicted RING-type ubiquitin ligase previously annotated as ATL31. Overexpression of ATL31 was confirmed to be responsible for the cni1-D phenotype, and a knock-out of this gene resulted in hypersensitivity to C/N conditions during post-germinative growth. The ATL31 protein was confirmed to contain ubiquitin ligase activity using an in vitro assay system. Moreover, removal of this ubiquitin ligase activity from the overexpressed protein resulted in the loss of the mutant phenotype. Taken together, these data demonstrated that CNI1/ATL31 activity is required for the plant C/N response during seedling growth transition.

Details

Title: CNI1/ATL31, a RING-type ubiquitin ligase that functions in the carbon/nitrogen response for growth phase transition in Arabidopsis seedlings
Authors/Creators: T. Sato (Author/Creator) - Hokkaido University
S. Maekawa (Author/Creator) - Hokkaido University
S. Yasuda (Author/Creator) - Hokkaido University
Y. Sonoda (Author/Creator) - Hokkaido University
E. Katoh (Author/Creator) - Institute of Agrobiological Sciences
T. Ichikawa (Author/Creator) - RIKEN
M. Nakazawa (Author/Creator) - RIKEN
M. Seki (Author/Creator) - RIKEN
K. Shinozaki (Author/Creator) - RIKEN
M. Matsui (Author/Creator) - RIKEN
D.B. Goto (Author/Creator) - Hokkaido University
A. Ikeda (Author/Creator) - Hokkaido University
J. Yamaguchi (Author/Creator) - Hokkaido University
Publication Details: The Plant Journal, Vol.60(5), pp.852-864
Publisher: Blackwell Publishing
Identifiers: 991005544651707891
Copyright: © 2009 Blackwell Publishing Ltd.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

30 Record Views

120 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.419 Jasmonic Acid
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science