Journal article
CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in barley
Journal of Integrative Plant Biology, Vol.64(3), pp.756-770
2022
Abstract
Nitrogen is a major determinant of grain yield and quality. As excessive use of nitrogen fertilizer leads to environmental pollution and high production costs, improving nitrogen use efficiency (NUE) is fundamental for a sustainable agriculture. Here, we dissected the role of the barley abnormal cytokinin response1 repressor 1 (HvARE1) gene, a candidate for involvement in NUE previously identified in a genome-wide association study, through natural variation analysis and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated gene editing. HvARE1 was predominantly expressed in leaves and shoots, with very low expression in roots under low nitrogen conditions. Agrobacterium-mediated genetic transformation of immature embryos (cv. Golden Promise) with single guide RNAs targeting HvARE1 generated 22 T0 plants, from which four T1 lines harbored missense and/or frameshift mutations based on genotyping. Mutant are1 lines exhibited an increase in plant height, tiller number, grain protein content, and yield. Moreover, we observed a 1.5- to 2.8-fold increase in total chlorophyll content in the flag leaf at the grain filling stage. Delayed senescence by 10–14 d was also observed in mutant lines. Barley are1 mutants had high nitrogen content in shoots under low nitrogen conditions. These findings demonstrate the potential of ARE1 in NUE improvement in barley.
Details
- Title
- CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in barley
- Authors/Creators
- S.D. Karunarathne (Author/Creator) - Murdoch UniversityY. Han (Author/Creator) - Department of Primary Industries and Regional DevelopmentX‐Q Zhang (Author/Creator)C. Li (Author/Creator) - Department of Primary Industries and Regional Development
- Publication Details
- Journal of Integrative Plant Biology, Vol.64(3), pp.756-770
- Publisher
- Blackwell Publishing Ltd
- Identifiers
- 991005544508607891
- Copyright
- © 2022 Institute of Botany, Chinese Academy of Sciences
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; Western Crop Genetics Alliance
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.54 Molecular & Cell Biology - Genetics
- 1.54.1401 Genome Editing
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Plant Sciences
- ESI research areas
- Plant & Animal Science