CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production

Anzu Okada; Taj Arndell; Nikolai Borisjuk; Niharika Sharma; Nathan S Watson-Haigh; Elise J. Tucker; Ute Baumann; Peter Langridge; Ryan Whitford

doi:10.1111/pbi.13106

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CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production

Journal article

Open access

Peer reviewed

CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production

Anzu Okada, Taj Arndell, Nikolai Borisjuk, Niharika Sharma, Nathan S Watson-Haigh, Elise J. Tucker, Ute Baumann, Peter Langridge and Ryan Whitford

Plant biotechnology journal, Vol.17(10), pp.1905-1913

2019

DOI: https://doi.org/10.1111/pbi.13106

PMID: 30839150

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Abstract

Biotechnology & Applied Microbiology

Life Sciences & Biomedicine

Plant Sciences

Science & Technology

The development and adoption of hybrid seed technology have led to dramatic increases in agricultural productivity. However, it has been a challenge to develop a commercially viable platform for the production of hybrid wheat (Triticum aestivum) seed due to wheat's strong inbreeding habit. Recently, a novel platform for commercial hybrid seed production was described. This hybridization platform utilizes nuclear male sterility to force outcrossing and has been applied to maize and rice. With the recent molecular identification of the wheat male fertility gene Ms1, it is now possible to extend the use of this novel hybridization platform to wheat. In this report, we used the CRISPR/Cas9 system to generate heritable, targeted mutations in Ms1. The introduction of biallelic frameshift mutations into Ms1 resulted in complete male sterility in wheat cultivars Fielder and Gladius, and several of the selected male-sterile lines were potentially non-transgenic. Our study demonstrates the utility of the CRISPR/Cas9 system for the rapid generation of male sterility in commercial wheat cultivars. This represents an important step towards capturing heterosis to improve wheat yields, through the production and use of hybrid seed on an industrial scale.

Details

Title: CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production
Authors/Creators: Anzu Okada - The University of Adelaide
Taj Arndell - The University of Adelaide
Nikolai Borisjuk - The University of Adelaide
Niharika Sharma - The University of Adelaide
Nathan S Watson-Haigh - The University of Adelaide
Elise J. Tucker - The University of Adelaide
Ute Baumann - The University of Adelaide
Peter Langridge - Univ Adelaide, Sch Agr Food & Wine, Urrbrae, SA, Australia
Ryan Whitford - The University of Adelaide
Publication Details: Plant biotechnology journal, Vol.17(10), pp.1905-1913
Publisher: Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
Number of pages: 9
Identifiers: 991005779529107891
Murdoch Affiliation: Centre for Crop and Food Innovation
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.54 Molecular & Cell Biology - Genetics; 1.54.1401 Genome Editing
Web Of Science research areas: Biotechnology & Applied Microbiology; Plant Sciences
ESI research areas: Plant & Animal Science