Logo image
Back
CYPA and CD147 expression analysis in human glioblastoma: Evidence for a correlation with reduced survival
Journal article   Open access   Peer reviewed

CYPA and CD147 expression analysis in human glioblastoma: Evidence for a correlation with reduced survival

V. Matthews, P.A. Candy, K.A. West, N.W. Knuckey, P. Robbins, T. Jeffcote and S. Boulos
Journal of Translational Science, Vol.4(5), pp.1-7
2018
DOI: https://doi.org/10.15761/JTS.1000249
pdf
CYPA and CD147 expression analysis in human glioblastoma - Evidence for a correlation with reduced survival.pdfDownloadView
Published (Version of Record) Open Access
url
Free to Read *No subscription requiredView

Abstract

Glioblastomas (GBMs) are fatal WHO grade IV astrocytomas with a median survival of 14.6 months. Immunohistochemical (IHC) studies indicate that cyclophilin A (CYPA), and its receptor CD147 are elevated in GBM, but the impacts on disease progression are poorly understood. We determined their occurrence in diffuse astrocytomas (WHO grades II-IV) using IHC and evaluated how gene expression affects patient outcomes in The Cancer Genome Atlas (TCGA) and REMBRANDT datasets (GBM and Low-Grade-Glioma: LGG). While IHC staining of CYPA was evident across all tumour grades, CD147 staining increased with tumour grade. However, while analysis of the TCGA grade II/III tumour dataset showed a trend towards high CD147 gene expression and reduced patient survival (HR=2.34, p=0.064, n=132), the results were not statistically significant. By contrast, analysis of the TCGA GBM dataset showed that high CYPA expression was significantly associated with reduced survival (HR=1.30, p=0.009; n=593). Furthermore, Kaplan-Meier analysis of both the TCGA and REMBRANDT (p=0.052; n=176) GBM datasets showed a comparable reduction in survival of about 3-months for patients with high CYPA expression. We found that CYPA gene amplification (≥2.2 copies) occurred in 72% of patients (REMBRANDT GBM dataset) and was correlated with reduced survival (log-rank p = 0.022). Finally, we observed a correlation between CYPA and CD147 gene expression in grade II/III (R=0.481, p=1.81x10-6) and grade IV (R=0.132; p=0.0012) tumours. Together, our data identify CYPA and CD147 as potential pathogenic determinants in glioblastoma.

Details

Metrics

54 File views/ downloads
41 Record Views
Logo image