Journal article
Candida albicans SOU1 encodes a sorbose reductase required forL-sorbose utilization
Yeast, Vol.22(12), pp.957-969
2005
Abstract
Previous work in our laboratory showed that L-sorbose utilization in Candida albicans is subject to a novel form of regulation which involves a reversible increase or decrease in the copy number of chromosome 5. Furthermore, the structural gene SOU1 is required for L-sorbose utilization and encodes a member of the short chain dehydrogenase family. However, the precise function of SOU1 was not known and neither was the pathway for L-sorbose utilization. We have now expressed SOU1 at a high level from a replicative plasmid having a constitutive ADH1 promoter and purified a version of Sou1p tagged with the FLAG epitope at the N-terminus. Sou1FLAGNp has a sorbose reductase activity which utilizes NADPH as a co-factor and converts L-sorbose to D-sorbitol. It can also less efficiently utilize fructose as a substrate with NADPH as a co-factor, converting fructose to mannitol. In agreement with prediction, the purified enzyme has a subunit molecular weight of 31 kDa and a pI of about 4.8. It probably consists of four identical subunits and has a pH optimum of 6.2. The L-sorbose utilization pathway in C. albicans probably converts L-sorbose to fructose-6-phosphate via D-sorbitol as an intermediate. The first step is catalysed by Sou1p. We also found that C. albicans extracts have a D-sorbitol-6-phosphate dehydrogenase activity, not encoded by SOU1, which utilizes NADP as a co-factor. This activity has not been described previously in yeasts and may be involved in the conversion of phosphorylated D-sorbitol to fructose-6-phosphate or glucose-6-phosphate.
Details
- Title
- Candida albicans SOU1 encodes a sorbose reductase required forL-sorbose utilization
- Authors/Creators
- J.R. Greenberg (Author/Creator)N.P. Price (Author/Creator)R.P. Oliver (Author/Creator)F. Sherman (Author/Creator)E. Rustchenko (Author/Creator)
- Publication Details
- Yeast, Vol.22(12), pp.957-969
- Publisher
- John Wiley and Sons Ltd
- Identifiers
- 991005542993907891
- Copyright
- © 2005 John Wiley & Sons, Ltd.
- Murdoch Affiliation
- Australian Centre for Necrotrophic Fungal Pathogens
- Language
- English
- Resource Type
- Journal article
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