Journal article
Candida albicans adhesion to human epithelial cells and polystyrene and formation of biofilm is reduced by sub-inhibitory Melaleuca alternifolia (tea tree) essential oil
Medical Mycology, Vol.50(8), pp.863-870
2012
Abstract
This study investigated the effects of the volatile terpene-rich oil from Melaleuca alternifolia (tea tree oil) on the formation of biofilms and the adhesion of C. albicans cells to both biotic and abiotic surfaces. Biofilm formation on polystyrene was significantly inhibited for 70% of the isolates at the lowest test concentration of 0.016% of tea tree oil (TTO) when quantified by XTT and 40% of isolates when measured by crystal violet staining. Adhesion to polystyrene, quantified by crystal violet staining, was significantly reduced for 3 isolates at 0.031%, 6 isolates at 0.062% and 0.125% and for all 7 isolates at 0.25% TTO. Reductions in adhesion were not due to loss of viability (at concentrations of ≤0.125%) or interactions between the TTO and polystyrene. Similarly, adhesion to buccal epithelial and HeLa cells was also significantly reduced in the presence of 0.0160.062% TTO. Treatment with 0.125% TTO, but not 0.062%, decreased the cell surface hydrophobicity of C. albicans, indicating one potential mechanism by which adhesion may be reduced. These data demonstrate that sub-inhibitory TTO reduces the adhesion of C. albicans to both human cells and polystyrene, inhibits biofilm formation and decreases cell surface hydrophobicity.
Details
- Title
- Candida albicans adhesion to human epithelial cells and polystyrene and formation of biofilm is reduced by sub-inhibitory Melaleuca alternifolia (tea tree) essential oil
- Authors/Creators
- A.N. Sudjana (Author/Creator) - The University of Western AustraliaC.F. Carson (Author/Creator) - The University of Western AustraliaK.C. Carson (Author/Creator) - Pathwest Laboratory MedicineT.V. Riley (Author/Creator) - The University of Western AustraliaK.A. Hammer (Author/Creator) - The University of Western Australia
- Publication Details
- Medical Mycology, Vol.50(8), pp.863-870
- Publisher
- Taylor & Francis
- Identifiers
- 991005541443207891
- Copyright
- © 2012 ISHAM.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
65 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 1 Clinical & Life Sciences
- 1.148 Medical Mycology
- 1.148.94 Antifungal Strategies
- Web Of Science research areas
- Infectious Diseases
- Mycology
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science