Files and links (1)
Published (Version of Record)CC BY-NC-ND V4.0, Open Access
Journal article
Candidate genes conferring ethylene-response in cultivated peanuts determined by BSA-seq and fine-mapping
The Crop journal, Vol.12(3), pp.856-865
2024
Abstract
Ethylene plays essential roles in plant growth, development and stress responses. The ethylene signaling pathway and molecular mechanism have been studied extensively in Arabidopsis and rice but limited in peanuts. Here, we established a sand-culture method to screen pingyangmycin mutagenized peanut lines based on their specific response to ethylene (“triple response“). An ethylene-insensitive mutant, inhibition of peanut hypocotyl elongation 1 (iph1), was identified that showed reduced sensitivity to ethylene in both hypocotyl elongation and root growth. Through bulked segregant analysis sequencing, a major gene related to iph1, named AhIPH1, was preliminarily mapped at the chromosome Arahy.01, and further narrowed to a 450-kb genomic region through substitution mapping strategy. A total of 7014 genes were differentially expressed among the ACC treatment through RNA-seq analysis, of which only the Arahy.5BLU0Q gene in the candidate mapping interval was differentially expressed between WT and mutant iph1. Integrating sequence variations, functional annotation and transcriptome analysis revealed that a predicated gene, Arahy.5BLU0Q, encoding SNF1 protein kinase, may be the candidate gene for AhIPH1. This gene contained two single-nucleotide polymorphisms at promoter region and was more highly expressed in iph1 than WT. Our findings reveal a novel ethylene-responsive gene, which provides a theoretical foundation and new genetic resources for the mechanism of ethylene signaling in peanuts
Details
- Title
- Candidate genes conferring ethylene-response in cultivated peanuts determined by BSA-seq and fine-mapping
- Authors/Creators
- Yanyan Tang - Qingdao Agricultural UniversityZhong Huang - Qingdao Agricultural UniversityShaohui Xu - Qingdao Agricultural UniversityWenjie Zhou - Qingdao Agricultural UniversityJianjun Ren - Qingdao Agricultural UniversityFuxin Yu - Qingdao Agricultural UniversityJingshan Wang - Qingdao Agricultural UniversityWujun Ma - Murdoch University, Centre for Crop and Food InnovationLixian Qiao - Qingdao Agricultural University
- Publication Details
- The Crop journal, Vol.12(3), pp.856-865
- Publisher
- Elsevier B.V. on behalf of KeAi Communications Co. Ltd.
- Identifiers
- 991005662061507891
- Copyright
- © 2024 Crop Science Society of China and Institute of Crop Science, CAAS.
- Murdoch Affiliation
- Food Futures Institute
- Language
- English
- Resource Type
- Journal article
Metrics
4 File views/ downloads
71 Record Views