Journal article
Carbohydrate coated fluorescent mesoporous silica particles for bacterial imaging
Colloids and Surfaces B: Biointerfaces, Vol.188, Article110751
2020
Abstract
This work investigated the synthesis of carbohydrate functionalized methylene blue doped amine grafted mesoporous silica nanoparticles (MB AMSN) and their application in bioimaging. A single-pot synthesis methodology was developed via a modified co-condensation sol-gel technique for simultaneous incorporation of the dye molecule in the nanoparticles, with amine grafting for subsequent functionalization. The obtained nanoparticles (∼ 450 nm) are mesoporous and have a high surface area (538 m2/g), pore-volume (0.3 cm3/g), showed excellent UV-vis absorbance, and dye encapsulation efficiency (> 75 %). These fluorescent nanoparticles were further functionalized with carbohydrate molecules before application as contrast agents in bacterial cells. In the present study, gram-positive (E. coli) and gram-negative (B. subtilis) bacteria were used as model organisms. Confocal laser microscopy results showed that the nanoparticles are highly fluorescent, and SEM of glucose conjugated MB doped nanoparticles indicated close interaction with E. coli with no toxicity observed towards either bacterial cells. The results demonstrate that by suitable surface functionalization, the methylene blue doped silica nanoparticles can be used as bioimaging agents.
Details
- Title
- Carbohydrate coated fluorescent mesoporous silica particles for bacterial imaging
- Authors/Creators
- H. Kirla (Author/Creator) - Murdoch UniversityL. Hughes (Author/Creator) - Murdoch UniversityD.J. Henry (Author/Creator) - Murdoch University
- Publication Details
- Colloids and Surfaces B: Biointerfaces, Vol.188, Article110751
- Publisher
- Elsevier
- Identifiers
- 991005540129807891
- Copyright
- © 2019 Elsevier B.V.
- Murdoch Affiliation
- Chemistry and Physics
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.41 Catalysts
- 2.41.148 Mesoporous Silica
- Web Of Science research areas
- Biophysics
- Chemistry, Physical
- Materials Science, Biomaterials
- ESI research areas
- Biology & Biochemistry