Journal article
Cell-type specific H+-ATPase activity enables root K+ retention and mediates acclimation to salinity
Plant Physiology, Vol.172, pp.2445-2448
2016
Abstract
While the importance of cell-type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal physiological mechanisms conferring higher sensitivity of apical root cells to salinity in barley. We show that salinity application to the root apex arrests root growth in a highly tissue- and treatment-specific manner. Although salinity-induced transient net Na+ uptake was about 4-fold higher in the root apex compared with the mature zone, mature root cells accumulated more cytosolic and vacuolar Na+ suggesting that higher sensitivity of apical cells to salt is not related to either enhanced Na+ exclusion or sequestration inside the root. Rather, the above differential sensitivity between the two zones originates from a 10-fold difference in K+ efflux between the mature zone and the apical region (much poorer in the root apex) of the root. Major factors contributing to this poor K+ retention ability are: (1) an intrinsically lower H+-ATPase activity in the root apex; (2) greater salt-induced membrane depolarization and (3) a higher ROS production under NaCl and a larger density of ROS-activated cation currents in the apex. Salinity treatment increased (2 to 5 fold) the content of 10 (out of 25 detected) amino acids in the root apex but not in the mature zone and changed the organic acid and sugar contents. The causal link between observed changes in the root metabolic profile and regulation of transporters activity is discussed.
Details
- Title
- Cell-type specific H+-ATPase activity enables root K+ retention and mediates acclimation to salinity
- Authors/Creators
- L. Shabala (Author/Creator) - University of TasmaniaJ. Zhang (Author/Creator) - University of TasmaniaI.I. Pottosin (Author/Creator) - Universidad de ColimaJ. Bose (Author/Creator) - Uinv. AdelaideM. Zhu (Author/Creator) - University of TasmaniaA.T. Fuglsang (Author/Creator) - University of CopenhagenA. Velarde-Buendia (Author/Creator) - Universidad de ColimaA. Massart (Author/Creator) - Universitat Autònoma de BarcelonaC.B. Hill (Author/Creator) - Murdoch UniversityU. Roessner (Author/Creator) - The University of MelbourneA. Bacic (Author/Creator) - The University of MelbourneH. Wu (Author/Creator) - University of TasmaniaE. Azzarello (Author/Creator) - University of FlorenceC. Pandolfi (Author/Creator) - New York University FlorenceM. Zhou (Author/Creator) - University of TasmaniaC. Poschenrieder (Author/Creator) - Universitat Autònoma de BarcelonaS. Mancuso (Author/Creator) - University of FlorenceS. Shabala (Author/Creator) - University of Tasmania
- Publication Details
- Plant Physiology, Vol.172, pp.2445-2448
- Publisher
- American Society of Plant Biologists
- Identifiers
- 991005540105307891
- Copyright
- © 2016 American Society of Plant Biologists
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science