Journal article
Cellular stress and p53-Associated apoptosis by Juniperus communis L. berry extract treatment in the human SH-SY5Y neuroblastoma cells
International Journal of Molecular Sciences, Vol.17(7)
2016
Abstract
Plant phenolics have shown to activate apoptotic cell death in different tumourigenic cell lines. In this study, we evaluated the effects of juniper berry extract (Juniperus communis L.) on p53 protein, gene expression and DNA fragmentation in human neuroblastoma SH-SY5Y cells. In addition, we analyzed the phenolic composition of the extract. We found that juniper berry extract activated cellular relocalization of p53 and DNA fragmentation-dependent cell death. Differentially expressed genes between treated and non-treated cells were evaluated with the cDNA-RDA (representational difference analysis) method at the early time point of apoptotic process when p53 started to be activated and no caspase activity was detected. Twenty one overexpressed genes related to cellular stress, protein synthesis, cell survival and death were detected. Interestingly, they included endoplasmic reticulum (ER) stress inducer and sensor HSPA5 and other ER stress-related genes CALM2 and YKT6 indicating that ER stress response was involved in juniper berry extract mediated cell death. In composition analysis, we identified and quantified low concentrations of fifteen phenolic compounds. The main groups of them were flavones, flavonols, phenolic acids, flavanol and biflavonoid including glycosides of quercetin, apigenin, isoscutellarein and hypolaetin. It is suggested that juniper berry extract induced the p53-associated apoptosis through the potentiation and synergism by several phenolic compounds.
Details
- Title
- Cellular stress and p53-Associated apoptosis by Juniperus communis L. berry extract treatment in the human SH-SY5Y neuroblastoma cells
- Authors/Creators
- T. Lantto (Author/Creator) - University of HelsinkiI. Laakso (Author/Creator) - University of HelsinkiH. Dorman (Author/Creator)T. Mauriala (Author/Creator) - University of HelsinkiR. Hiltunen (Author/Creator) - University of HelsinkiS. Kõks (Author/Creator) - University of TartuA. Raasmaja (Author/Creator) - University of Tartu
- Publication Details
- International Journal of Molecular Sciences, Vol.17(7)
- Publisher
- MDPI AG
- Identifiers
- 991005540296907891
- Copyright
- © 2019 MDPI
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.16 Phytochemicals
- 3.16.314 Essential Oil
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry