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Cellulose Hydrolysis of Mask Waste Using Aspergillus niger and Eco-Friendly Microwave Pretreatment
Journal article   Open access   Peer reviewed

Cellulose Hydrolysis of Mask Waste Using Aspergillus niger and Eco-Friendly Microwave Pretreatment

Adam Gilbran, Syahraini Nafilah, Afina Rista Layalia, Wifqul Muna Arsyad, Andi Darmawan, Risqi Prastianto Setiawan, Risnu Irviandi, Endang Kusdiyantini, Aida Habibah Nurauliyaa, Martin Anda, …
Jurnal Presipitasi, Vol.22(3), pp.993-1001
2025
DOI: https://doi.org/10.14710/presipitasi.v22i3.993-1001
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CC BY-SA V4.0,  Open Access

Abstract

aspergillus niger hydrolysis mask waste
The management of used medical mask waste has become a significant issue due to the increased volume of waste during and after the pandemic. Medical mask waste contains cellulose compounds that can be converted into derivatives such as glucose, which are then processed into bioethanol as an alternative energy source. This study aims to hydrolyse medical mask waste using cellulase enzymes from Aspergillus Niger to produce glucose. The cellulase enzyme composition was varied (5 ml, 15 ml, and 25 ml) to determine the optimal hydrolysis conditions. The glucose produced was measured using DNS reagent assay with spectrophotometry at a wavelength of 540 nm. The highest amount of glucose was obtained under optimal conditions with 25 ml of cellulase enzyme after 48 hours of hydrolysis, amounting to 88.16 ppm. Subsequently, the glucose from hydrolysis was fermented using Saccharomyces cerevisiae, and the fermentation product was analysed for ethanol concentration using GC-FID. The products of fermentation ware 0.017% ethanol concentration from mask waste fermentation. Hydrolysis is an environmentally friendly alternative solution for handling mask waste.

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