Journal article
Cereal DNA: A rapid high-throughput extraction method for marker assisted selection
Plant Molecular Biology Reporter, Vol.18(4), pp.357-360
2000
Abstract
A rapid, automated and novel method is presented to extract DNA suitable for polymerase chain reaction (PCR) from small amounts of cereal leaf tissue in a high-throughput cost-effective way. The method uses a 96-well plate in which leaf samples are frozen, mechanically crushed using a matrix mill, macerated in alkali and subsequently neutralized. The method was used routinely with barley and wheat leaf samples and the extracted material was used to screen for specific traits of interests, including barley yellow dwarf virus resistance and β-amylase activity in barley and stem rust resistance in wheat. This system allows complete PCR analysis of 384 seedlings or more by one person in a day.
Details
- Title
- Cereal DNA: A rapid high-throughput extraction method for marker assisted selection
- Authors/Creators
- M. Paris (Author/Creator)M. Carter (Author/Creator)
- Publication Details
- Plant Molecular Biology Reporter, Vol.18(4), pp.357-360
- Publisher
- Springer New York
- Identifiers
- 991005545304207891
- Copyright
- 2000 International Society for Plant Molecular Biology
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Biochemical Research Methods
- Plant Sciences
- ESI research areas
- Plant & Animal Science