Journal article
Changes in protein biosynthesis during the differentiation of Pisolithus - Eucalyptus grandis ectomycorrhiza
Canadian Journal of Botany, Vol.74(4), pp.553-560
1996
Abstract
Protein biosynthesis in Pisolithus - Eucalyptus grandis ectomycorrhiza was related to the stage of ectomycorrhizal development using two-dimensional polyacrylamide gel electrophoresis of proteins labelled by in vivo incorporation of 35S radiolabelled amino acids. Nineteen-day-old seedlings were radiolabelled and the primary root was divided into 1-cm segments. With increasing distance from the tip of the primary root, the lateral roots developed as follows: segment 1, no lateral tips; segment 2, three lateral tips, 1-4 days old; segment 3, five lateral tips, 3-8 days old; segment 4, five lateral tips, 7-12 days old. Six-day-old ectomycorrhizas were fully formed with a mantle and Hartig net. During ectomycorrhizal development, there was a decrease in all plant proteins and differential accumulation of fungal proteins. The apical segment of the primary root had a biosynthesis profile very similar to that of noninoculated roots By contrast, the other segments of the primary root, with attached lateral roots, had biosynthesis profiles that were similar to those of the free-living hyphae. Thus, plant biosynthesis was shown to be predominantly associated with the primary root meristem. The domination of the fungal partner in the protein biosynthesis of developing ectomycorrhiza is probably a consequence of stimulated fungal growth and the corresponding decrease in plant meristematic activity. Ectomycorrhizal development was associated with a differential accumulation of fungal polypeptides and the appearance of a group of symbiosis-related acid fungal polypeptides between 27 and 37 kDa. As the polypeptides were present in a similar magnitude throughout ectomycorrhizal development (lateral tips 1-12 days old), it is suggested that they function as structural proteins associated with mantle formation.
Details
- Title
- Changes in protein biosynthesis during the differentiation of Pisolithus - Eucalyptus grandis ectomycorrhiza
- Authors/Creators
- T. Burgess (Author/Creator)B. Dell (Author/Creator)
- Publication Details
- Canadian Journal of Botany, Vol.74(4), pp.553-560
- Publisher
- NRC Research Press
- Identifiers
- 991005544881207891
- Murdoch Affiliation
- School of Biological and Environmental Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
401 File views/ downloads
58 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.488 Mycorrhizal Symbiosis
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science