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Changing topography of the RPE resulting from experimentally induced rapid eye growth
Journal article   Open access   Peer reviewed

Changing topography of the RPE resulting from experimentally induced rapid eye growth

P.A. Fleming, A.M. Harman and L.D. Beazley
Visual Neuroscience, Vol.14(03), pp.449-461
1997
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Abstract

The retinal pigment epithelium (RPE) of the quokka wallaby, Setonix brachyurus, grows and changes throughout life. To investigate factors that determine changes in the quokka RPE, we have examined topography of this tissue in experimentally enlarged eyes. Unilateral eyelid suture was conducted at the time of normal eye opening, postnatal day (P) 110, and animals were examined at 1 or 1 1/4 years of age. The numbers and densities of RPE cells and the extent of multinucleation were compared with those in normal animals. Eyelid suture resulted in a 9.8% and 17.4% increase in retinal area at 1 and 1 1/4 years, respectively; a significant degree of myopia was associated with this enlargement. Cell density topography in experimental eyes was not the same as in controls. Cells from central retina were disproportionately larger in the experimental than control eyes. However, the RPE cell topography in sutured eyes was not the same as that of aged retinae of a similar size. Notably, in sutured eyes there was no development of the high or highest cell densities seen in equatorial and temporal central RPE in aged retinae, respectively. Furthermore, the degree of cell enlargement in peripheral regions was slight compared with that observed in similar-sized, aged retinae. There was no increase in RPE cell number; rather, average cell area increased accompanied by no change or a slight decrease in RPE thickness. Consequently, overall volume of cells did not change significantly. The large number of multinucleate cells normally seen n aged animals was not observed in experimentally enlarged eyes, implying that an increase in cell volume may be the trigger for multinucleation.

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1 Clinical & Life Sciences
1.36 Ophthalmology
1.36.813 Vision Disorders
Web Of Science research areas
Neurosciences
Ophthalmology
ESI research areas
Neuroscience & Behavior
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