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Characterisation and quantitation of mutant and wild-type genomes of Hardenbergia mosaic virus isolates co-infecting a wild plant of Hardenbergia comptoniana
Journal article   Peer reviewed

Characterisation and quantitation of mutant and wild-type genomes of Hardenbergia mosaic virus isolates co-infecting a wild plant of Hardenbergia comptoniana

S.J. Wylie and M.G.K. Jones
Archives of Virology, Vol.156(7), pp.1251-1255
2011
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Abstract

Deep sequencing of the polyadenylated transcriptome of a wild plant of Hardenbergia comptoniana was used to distinguish with high support complete genome sequences of two distinct isolates of Hardenbergia mosaic virus (HarMV) co-infecting it. Isolates shared 82.0% nucleotide (nt) and 86.7% amino acid (aa) identity. Isolate 57.1 (9,628 nt) had a deletion of 17 contiguous aa within the conserved core region of the coat protein (CP), while isolate 57.2 (9,675 nt) had a wild-type CP. Overall, HarMV genomes accounted for 10.69% of total transcripts sequenced, and the mutant virus genome was 23.1% more highly expressed than the wild-type genome.

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Citation topics
3 Agriculture, Environment & Ecology
3.267 Virology - Plant
3.267.356 Plant Virus Interactions
Web Of Science research areas
Virology
ESI research areas
Microbiology
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