Characterisation of an extracellular serine protease gene (naspgene) from Dermatophilus congolensis

A. Garcia-Sanchez; R. Cerrato; J. Larrasa; N.C. Ambrose; A. Parra; J.M. Alonso; M. Hermoso-de-Mendoza; J.M. Rey; O.M. Mine; P.R. Carnegie; T.M. Ellis; A.M. Masters; A.D. Pemberton; J. Hermoso-de-Mendoza

doi:10.1016/S0378-1097(03)00958-3

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Characterisation of an extracellular serine protease gene (naspgene) from Dermatophilus congolensis

Journal article

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Characterisation of an extracellular serine protease gene (naspgene) from Dermatophilus congolensis

A. Garcia-Sanchez, R. Cerrato, J. Larrasa, N.C. Ambrose, A. Parra, J.M. Alonso, M. Hermoso-de-Mendoza, J.M. Rey, O.M. Mine, P.R. Carnegie, …

FEMS Microbiology Letters, Vol.231(1), pp.53-57

2004

DOI: https://doi.org/10.1016/S0378-1097(03)00958-3

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Abstract

A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp internal fragment of the gene, which was finally characterised by inverse PCR as the first full-length sequenced serine protease gene (nasp) from Dermatophilus congolensis. The deduced amino acid sequence of this enzyme, probably involved in the pathogenesis of dermatophilosis, links it to the subtilisin family of proteases. © 2004 Federation of European Microbiological Societies.

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Title: Characterisation of an extracellular serine protease gene (naspgene) from Dermatophilus congolensis
Authors/Creators: A. Garcia-Sanchez (Author/Creator) - Universidad de Extremadura
R. Cerrato (Author/Creator) - Universidad de Extremadura
J. Larrasa (Author/Creator) - Microbiology Department, Laboratorios Larrasa, Jerez de los Caballeros, Badajoz, Spain
N.C. Ambrose (Author/Creator) - Centre for Tropical Veterinary Medicine, Royal (Dick) School of Veterinary Studies, Edinburgh, UK
A. Parra (Author/Creator) - Universidad de Extremadura
J.M. Alonso (Author/Creator) - Universidad de Extremadura
M. Hermoso-de-Mendoza (Author/Creator) - Universidad de Extremadura
J.M. Rey (Author/Creator) - Universidad de Extremadura
O.M. Mine (Author/Creator)
P.R. Carnegie (Author/Creator) - Murdoch University
T.M. Ellis (Author/Creator) - Agriculture Fisheries and Conservation Department
A.M. Masters (Author/Creator) - Animal Health Laboratories, Department of Agriculture of Western Australia, South Perth, WA, Australia
A.D. Pemberton (Author/Creator) - Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, Edinburgh, UK
J. Hermoso-de-Mendoza (Author/Creator) - Universidad de Extremadura
Publication Details: FEMS Microbiology Letters, Vol.231(1), pp.53-57
Publisher: Blackwell Publishing
Identifiers: 991005540947807891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.243 Kidney Diseases; 1.243.2150 Renal Infections
Web Of Science research areas: Microbiology
ESI research areas: Microbiology