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Journal article
Characterization and Genomic Analysis of Escherichia coli O157:H7 Phage UAE_MI-01 Isolated from Birds
International journal of molecular sciences, Vol.23(23), 14846
2022
PMID: 36499178
Abstract
Verotoxin-producing Escherichia coli O157:H7 is responsible for the majority of foodborne outbreaks worldwide and may lead to death. Bacteriophages are natural killers of bacteria. All previously reported E. coli O157:H7 phages were isolated from ruminants or swine. Here, we report for the first time a phage isolated from bird feces in the United Arab Emirates (UAE), designated as UAE_MI-01, indicating birds as a good source of phages. Thus, phages could be a tool for predicting the presence of the host bacteria in an animal or the environment. UAE_MI-01 was found to be a lytic phage that was stable at wide ranges of pH, temperature, and chemical disinfectants, and with a burst size of almost 100 plaque-forming units per host cell after a latent period of 20 min and an adsorption rate constant (K) of 1.25 × 10−7 mL min−1. The phage genome was found to be 44,281 bp long with an average GC content of 54.7%. The presence of the phage indicates the presence of the host cell E. coli O157:H7 in wild birds. Therefore, other birds, mainly poultry, could be also investigated for the presence of this pathogenic bacterium. To the best of our knowledge, this is the first report of an E. coli O157:H7 bacteriophage isolated from a bird.
Details
- Title
- Characterization and Genomic Analysis of Escherichia coli O157:H7 Phage UAE_MI-01 Isolated from Birds
- Authors/Creators
- Mohamad Ismail Sultan-Alolama - Zayed UniversityAmr Amin - United Arab Emirates UniversityKhaled A El-Tarabily - Murdoch University, Centre for Terrestrial Ecosystem Science and SustainabilityRanjit Vijayan - United Arab Emirates University
- Publication Details
- International journal of molecular sciences, Vol.23(23), 14846
- Publisher
- MDPI
- Grant note
- 12S060 / United Arab Emirates University 31R174 / United Arab Emirates University
- Identifiers
- 991005585962807891
- Copyright
- © 2022 by the authors.
- Murdoch Affiliation
- Harry Butler Institute
- Language
- English
- Resource Type
- Journal article
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.42 Bacteriology
- 1.42.1349 Bacteriophage
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry