Journal article
Characterization and recognition of Brachyspira hampsonii sp. Nov., a novel intestinal spirochete that is pathogenic to pigs
Journal of Clinical Microbiology, Vol.54(12), pp.2942-2949
2016
Abstract
Swine dysentery (SD) is a mucohemorrhagic colitis of swine classically caused by infection with the intestinal spirochete Brachyspira hyodysenteriae. Since around 2007, cases of SD have occurred in North America associated with a different strongly beta-hemolytic spirochete that has been molecularly and phenotypically characterized and provisionally named "Brachyspira hampsonii." Despite increasing international interest, B. hampsonii is currently not recognized as a valid species. To support its recognition, we sequenced the genomes of strains NSH-16T, NSH-24, and P280/1, representing B. hampsonii genetic groups I, II, and III, respectively, and compared them with genomes of other valid Brachyspira species. The draft genome of strain NSH-16T has a DNA G+C content of 27.4% and an approximate size of 3.2 Mb. Genomic indices, including digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI), and average amino acid identity (AAI), clearly differentiated B. hampsonii from other recognized Brachyspira species. Although discriminated genotypically, the three genetic groups are phenotypically similar. By electron microscopy, cells of different strains of B. hampsonii measure 5 to 10 μmby 0.28 to 0.34 μm, with one or two flat curves, and have 10 to 14 periplasmic flagella inserted at each cell end. Using a comprehensive evaluation of genotypic (gene comparisons and multilocus sequence typing and analysis), genomic (dDDH, ANI, and AAI) and phenotypic (hemolysis, biochemical profiles, protein spectra, antibiogram, and pathogenicity) properties, we classify Brachyspira hampsonii sp. Nov. as a unique species with genetically diverse yet phenotypically similar genomovars (I, II, and III). We designate the type strain NSH-16 (+ATCC BAA-2463+NCTC 13792).
Details
- Title
- Characterization and recognition of Brachyspira hampsonii sp. Nov., a novel intestinal spirochete that is pathogenic to pigs
- Authors/Creators
- N.S. Mirajkar (Author/Creator)N.D. Phillips (Author/Creator)T. La (Author/Creator)D.J. Hampson (Author/Creator)C.J. Gebhart (Author/Creator)
- Publication Details
- Journal of Clinical Microbiology, Vol.54(12), pp.2942-2949
- Publisher
- American Society for Microbiology
- Identifiers
- 991005545187607891
- Copyright
- © 2016, American Society for Microbiology.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
152 File views/ downloads
45 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.248 Sexually Transmitted Infections
- 1.248.2445 Lawsonia Intracellularis
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology