Journal article
Characterization of Giardia isolates using a non-radiolabeled DNA probe, and correlation with the results of isoenzyme analysis
American Journal of Tropical Medicine and Hygiene, Vol.40(6), pp.629-637
1989
Abstract
Forty-seven isolates, identified morphologically as Giardia duodenalis, were compared by restriction endonuclease analysis of DNA with hybridization to a non-radiolabeled probe. Seven schizodemes were distinguished, compared to 15 zymodemes identified by isoenzyme electrophoresis. Despite the greater sensitivity of isoenzyme electrophoresis, DNA analysis did detect previously unsuspected variations between isolates in 1 zymodeme. Eighteen different genetic groups were detected among the 49 isolates by isoenzyme and DNA analyses. Genetic differences between groups, calculated from DNA restriction fragment variation, were significantly correlated with differences calculated from allozyme variation. This correlation between the 2 techniques suggests that G. duodenalis consists of a complex of genetically diverse clones. Such a genetic structure has important implications for the taxonomy of Giardia and the epidemiology of giardiasis.
Details
- Title
- Characterization of Giardia isolates using a non-radiolabeled DNA probe, and correlation with the results of isoenzyme analysis
- Authors/Creators
- B.P. Meloni (Author/Creator)A.J. Lymbery (Author/Creator)R.C.A. Thompson (Author/Creator)
- Publication Details
- American Journal of Tropical Medicine and Hygiene, Vol.40(6), pp.629-637
- Publisher
- American Society of Tropical Medicine and Hygiene
- Identifiers
- 991005545169207891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.246 Diarrheal Diseases
- 1.246.985 Cryptosporidium
- Web Of Science research areas
- Public, Environmental & Occupational Health
- Tropical Medicine
- ESI research areas
- Clinical Medicine