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Journal article
Characterization of ‘QTL-hotspot’ introgression lines reveals physiological mechanisms and candidate genes associated with drought adaptation in chickpea
Journal of Experimental Botany, Vol.73(22), erac348
2022
Abstract
‘QTL-hotspot’ is a genomic region on linkage group 04 (CaLG04) in chickpea (Cicer arietinum) that harbours major-effect quantitative trait loci (QTLs) for multiple drought-adaptive traits, and it therefore represents a promising target for improving drought adaptation. To investigate the mechanisms underpinning the positive effects of ‘QTL-hotspot’ on seed yield under drought, we introgressed this region from the ICC 4958 genotype into five elite chickpea cultivars. The resulting introgression lines (ILs) and their parents were evaluated in multi-location field trials and semi-controlled conditions. The results showed that the ‘QTL-hotspot’ region improved seed yield under rainfed conditions by increasing seed weight, reducing the time to flowering, regulating traits related to canopy growth and early vigour, and enhancing transpiration efficiency. Whole-genome sequencing data analysis of the ILs and parents revealed four genes underlying the ‘QTL-hotspot’ region associated with drought adaptation. We validated diagnostic KASP markers closely linked to these genes using the ILs and their parents for future deployment in chickpea breeding programs. The CaTIFY4b-H2 haplotype of a potential candidate gene CaTIFY4b was identified as the superior haplotype for 100-seed weight. The candidate genes and superior haplotypes identified in this study have the potential to serve as direct targets for genetic manipulation and selection for chickpea improvement.
Details
- Title
- Characterization of ‘QTL-hotspot’ introgression lines reveals physiological mechanisms and candidate genes associated with drought adaptation in chickpea
- Authors/Creators
- R. Barmukh (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsM. Roorkiwal (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsG.P. Dixit (Author/Creator) - Indian Institute of Pulses ResearchP. Bajaj (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsJ. Kholova (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsM.R. Smith (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsA. Chitikineni (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsC. Bharadwaj (Author/Creator) - Indian Agricultural Research InstituteS.M. Sreeman (Author/Creator)A. Rathore (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsS. Tripathi (Author/Creator) - Indian Agricultural Research InstituteM. Yasin (Author/Creator) - Jawaharlal Nehru Krishi Vishwa VidyalayaA.G. Vijayakumar (Author/Creator) - UAS-Dharwad Regional Agricultural Research Station, Vijayapura, India.S. Rao Sagurthi (Author/Creator)K.H.M. Siddique (Author/Creator) - The University of Western AustraliaR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsC. Foyer (Author/Creator)
- Publication Details
- Journal of Experimental Botany, Vol.73(22), erac348
- Publisher
- Oxford University Press
- Identifiers
- 991005542087807891
- Copyright
- © 2022 The Authors.
- Murdoch Affiliation
- Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science