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Characterization of allergens of the cat flea, Ctenocephalides felis: detection and frequency of IgE antibodies in canine sera
Journal article   Peer reviewed

Characterization of allergens of the cat flea, Ctenocephalides felis: detection and frequency of IgE antibodies in canine sera

W.K. Greene, R.L. Carnegie, S.E. Shaw, R.C.A. Thompson and W.J. Penhale
Parasite Immunology, Vol.15(2), pp.69-74
1993
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Abstract

Flea allergens, fractionated by polyacrylamide gel electrophoresis and transferred to nitrocellulose, were identified using 20 flea-allergic dog sera in an enhanced chemiluminescent assay for canine IgE antibodies. At least 15 different flea components in the molecular weight range of 14-150 K bound IgE and every serum demonstrated a different pattern of binding. Three of the components with apparent molecular weights of 25, 40 and 58 K were each bound by at least 40% of the sera. No reactivity was seen when normal dog sera were used. These results demonstrate a greater number of flea allergens and a far greater diversity of the IgE antibody response to flea allergens than has previously been described, and suggest that immediate hypersensitivity may be an important mechanism in the pathogenesis of canine flea allergy.

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Source: InCites

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Citation topics
1 Clinical & Life Sciences
1.65 Allergy
1.65.1091 Atopic Dermatitis
Web Of Science research areas
Immunology
Parasitology
ESI research areas
Immunology
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