Journal article
Characterization of microsatellites and development of chromosome specific STMS markers in bread wheat
Plant Molecular Biology Reporter, Vol.18(1), pp.5-16
2000
Abstract
Microsatellites, or simple sequence repeats (SSRs), have become the markers of choice for genetic studies with many crop species including wheat. Currently an international effort is underway to enrich the repertoire of available sequence tagged microsatellite site (STMS) markers in wheat. As a part of this effort, we have sequenced 43 clones obtained from a microsatellite-enriched wheat genomic library; 34 clones contained 41 different microsatellites. These microsatellites (mono-, di-, tri- nucleotide repeats) were classified as 19 simple perfect, 18 simple imperfect and 4 compound imperfect types. Dinucleotide repeats were the most abundant (70%). Primer pairs for only 16 microsatellites could be designed, since the flanking sequences of the others were either too short or were otherwise not suitable for designing the microsatellite specific primers. Microsatellite loci of the expected size and polymorphism were successfully amplified from 15 of these 16 primer pairs using three wheat varieties. 14 loci detected by 12 out of the 15 functional primer pairs were assigned to 11 specific chromosomes.
Details
- Title
- Characterization of microsatellites and development of chromosome specific STMS markers in bread wheat
- Authors/Creators
- R.K. Varshney (Author/Creator) - Chaudhary Charan Singh UniversityA. Kumar (Author/Creator) - Chaudhary Charan Singh UniversityH.S. Balyan (Author/Creator) - Chaudhary Charan Singh UniversityJ.K. Roy (Author/Creator) - Chaudhary Charan Singh UniversityM. Prasad (Author/Creator) - Chaudhary Charan Singh UniversityP.K. Gupta (Author/Creator) - Chaudhary Charan Singh University
- Publication Details
- Plant Molecular Biology Reporter, Vol.18(1), pp.5-16
- Publisher
- Springer New York
- Identifiers
- 991005540548907891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Biochemical Research Methods
- Plant Sciences
- ESI research areas
- Plant & Animal Science