Characterization of normal canine anterior cruciate ligament-associated synoviocytes

Sunil C. Vasanjee; Daniel Paulsen; Giselle Hosgood; Sandra O. Robinson; Mandi J. Lopez

doi:10.1002/jor.20552

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Characterization of normal canine anterior cruciate ligament-associated synoviocytes

Journal article

Peer reviewed

Characterization of normal canine anterior cruciate ligament-associated synoviocytes

Sunil C. Vasanjee, Daniel Paulsen, Giselle Hosgood, Sandra O. Robinson and Mandi J. Lopez

Journal of orthopaedic research, Vol.26(6), pp.809-815

2008

DOI: https://doi.org/10.1002/jor.20552

PMID: 18203201

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Abstract

anterior cruciate ligament

synoviocytes

canine

animal model

immunohistochemistry

This study was designed to identify and quantify synoviocyte phenotypes enveloping the canine anterior cruciate ligament (ACL) to test the hypothesis that there are at least two synoviocyte phenotypes, each with distinct quantities and topographical distributions. CD18 and HSP25 epitopes were colocalized in the synovium of 10 normal canine ACLs. Sagittal sections were prepared from medial, central, and lateral aspects of each ACL and phenotypes were quantified in the proximal, middle, and distal aspects of each section. Distinct synoviocyte populations stained positive for CD18 (CD18+) or HSP25 (HSP25+), and a small population of cells stained for both epitopes (DS+). The proportion (mean ± SEM) of HSP25+ synoviocytes (57% ± 7.5%) was significantly greater than the proportion of CD18+ synoviocytes (27% ± 8.2%), which was significantly greater than the proportion of DS+ synoviocytes (16% ± 3.5%). Reverse transcriptase polymerase chain reaction (RT-PCR), Western blot analysis, and immunoelectron microscopy confirmed the presence of CD18 and HSP25 epitopes in the canine ACL. Identification and quantification of ACL synoviocytes may serve as the foundation for future studies involving ACL disease or reconstruction.

Details

Title: Characterization of normal canine anterior cruciate ligament-associated synoviocytes
Authors/Creators: Sunil C. Vasanjee - Louisiana State University
Daniel Paulsen - Louisiana State University
Giselle Hosgood - Louisiana State University
Sandra O. Robinson - Louisiana State University
Mandi J. Lopez - Louisiana State University
Publication Details: Journal of orthopaedic research, Vol.26(6), pp.809-815
Publisher: Wiley
Number of pages: 7
Grant note: K01 AR002174-05; K01 AR002174-06 / NIAMS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) K01AR002174 / NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS)
Identifiers: 991005592650707891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.34 Orthopedics; 1.34.440 Anterior Cruciate Ligament
Web Of Science research areas: Orthopedics
ESI research areas: Clinical Medicine