Journal article
Characterization of seed proteome in Brachypodium distachyon
Journal of Cereal Science, Vol.52(2), pp.177-186
2010
Abstract
Brachypodium distachyon, an emerging model plant system for some economically important temperate grasses such as wheat, barley and switchgrass, has recently caught wide attention in modern biological research. In the current study, the glutenin, albumin and globulin components of 13 B. distachyon accessions were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) followed by peptide mass finger printing (PMF) and MS/MS protein identification. Abundant wheat low molecular weight glutenin subunit (LMW-GS) like proteins and a few high molecular weight glutenin subunits (HMW-GS) with low expression level were detected in B. distachyon. A total of 18 storage proteins and 15 albumin proteins were identified through PMF and MS/MS. The results demonstrated that the major seed storage proteins in B. distachyon are wheat LMW-GS like proteins and globulins. The identified albumins and globulins were mostly various enzymes that were classified into five groups according to their functions. The 2-DE spot distribution and MS results suggested that post-translational modifications (PTMs) such as phosphorylations and glycosylations are common phenomena in B. distachyon seed proteome.
Details
- Title
- Characterization of seed proteome in Brachypodium distachyon
- Authors/Creators
- K. Wang (Author/Creator) - Capital Normal UniversityX. Han (Author/Creator) - Capital Normal UniversityK. Dong (Author/Creator) - Capital Normal UniversityL. Gao (Author/Creator) - Capital Normal UniversityH. Li (Author/Creator) - Institute of Crop SciencesW. Ma (Author/Creator) - Agriculture and FoodY. Yan (Author/Creator) - Capital Normal UniversityX. Ye (Author/Creator) - Institute of Crop Sciences
- Publication Details
- Journal of Cereal Science, Vol.52(2), pp.177-186
- Publisher
- Academic Press
- Identifiers
- 991005545416207891
- Copyright
- © 2010 Elsevier Ltd
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.99 Cereal Starch Properties
- Web Of Science research areas
- Food Science & Technology
- ESI research areas
- Agricultural Sciences