Journal article
Characterization of staphylococcal cassette chromosome mec elements from methicillin-resistant Staphylococcus pseudintermedius infections in Australian animals
mSphere, Vol.3(6), pp.e00491-18
2018
Abstract
We examined the oxacillin resistance phenotype and genomic structure of staphylococcal cassette chromosome mec (SCCmec) elements from 77 veterinary methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates. Isolates were characterized by oxacillin broth microdilution, whole-genome sequencing, and bioformatics analysis. Five previously described SCCmec elements, and a sixth novel element, were identified: SCCmec III (also known as II-III), ΨSCCmec57395, and SCCmecNA45 (a SCCmec VII variant), all previously described in MRSP, and SCCmec IVg and SCCmec VT, previously described in both methicillin-resistant Staphylococcus aureus (MRSA) and MRSP. The sixth element was novel and found among nine geographically clustered isolates. This novel pseudostaphylococcal cassette chromosome (ΨSCCmecKW21) contained a class A mec gene complex but lacked ccr genes. It also harbored heavy metal (cadmium) resistance determinants. The median oxacillin MIC values among ΨSCCmecKW21, SCCmec III, and SCCmec VT isolates were significantly higher than those determined for the SCCmecNA45 VII variant isolates and ΨSCCmec57395 and SCCmec IVg isolates. ΨSCCmecKW21 was found exclusively in sequence type 497 (ST497), an MRSP clone that is locally successful in Victoria, Australia. Future studies are necessary to determine if this clone has disseminated further afield and if ΨSCCmecKW21 has moved into other MRSP lineages or staphylococcal species.
Details
- Title
- Characterization of staphylococcal cassette chromosome mec elements from methicillin-resistant Staphylococcus pseudintermedius infections in Australian animals
- Authors/Creators
- K.A. Worthing (Author/Creator) - Peter Doherty InstituteS. Schwendener (Author/Creator) - University of BernV. Perreten (Author/Creator) - University of BernS. Saputra (Author/Creator) - The University of AdelaideG.W. Coombs (Author/Creator) - Murdoch UniversityS. Pang (Author/Creator) - Murdoch UniversityM.R. Davies (Author/Creator) - Peter Doherty InstituteS. Abraham (Author/Creator) - Murdoch UniversityD.J. Trott (Author/Creator) - The University of AdelaideJ.M. Norris (Author/Creator) - The University of SydneyP.D. Fey (Author/Creator)
- Publication Details
- mSphere, Vol.3(6), pp.e00491-18
- Publisher
- American Society for Microbiology
- Identifiers
- 991005541355307891
- Copyright
- © 2018 Worthing et al.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
80 File views/ downloads
61 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.23 Antibiotics & Antimicrobials
- 1.23.173 MRSA and VRE
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology