Characterization of the in vitro responses of equine cecal longitudinal smooth muscle to endothelin-1

Ramaswamy M Chidambaram; Susan C Eades; Rustin M Moore; Giselle Hosgood; Changaram S Venugopal

doi:10.2460/ajvr.2005.66.1202

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Characterization of the in vitro responses of equine cecal longitudinal smooth muscle to endothelin-1

Journal article

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Characterization of the in vitro responses of equine cecal longitudinal smooth muscle to endothelin-1

Ramaswamy M Chidambaram, Susan C Eades, Rustin M Moore, Giselle Hosgood and Changaram S Venugopal

American journal of veterinary research, Vol.66(7), pp.1202-1208

2005

DOI: https://doi.org/10.2460/ajvr.2005.66.1202

PMID: 16111159

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Abstract

Objective To characterize the in vitro response of equine cecal longitudinal smooth muscle (CLSM) to endothelin (ET)-1 and assess the role of ETA and ETB receptors in those ET-1–induced responses. Animals 36 horses without gastrointestinal tract disease. Procedure To determine cumulative concentrationresponse relationships, CLSM strips were suspended in tissue baths containing graded concentrations of ET-1 (10–9 to 10–6M) with or without BQ-123 (ETA receptor antagonist); with or without IRL-1038 (ETB receptor antagonist); or with both antagonists at concentrations of 10–9, 10–7, and 10–5M. To determine the percentage change in baseline tension of CLSM, the areas under the curve during the 3-minute periods before and after addition of each dose were compared . Also, the effects of ET-1 and a combination of selective ETA and ETB receptor antagonists on electrically evoked contractions were studied. Results ET-1 caused sustained increases in CLSM tension in a concentration-dependent manner. Contractile responses to ET-1 were not significantly inhibited by either BQ-123 or IRL-1038 alone at any concentration; however, responses were significantly inhibited by exposure to the antagonists together at a concentration of 10–5M. Electrical field stimulation did not change the spontaneous contractile activity of CLSM and did not significantly alter the tissue response to ET-1, BQ-123, or IRL-1038. Conclusions and Clinical Relevance Results indicated that ET-1 has a contractile effect on equine CLSM that is mediated via ETA and ETB receptors. In vitro spontaneous contractions of equine CLSM apparently originate in the smooth muscle and not the enteric nervous system.

Details

Title: Characterization of the in vitro responses of equine cecal longitudinal smooth muscle to endothelin-1
Authors/Creators: Ramaswamy M Chidambaram
Susan C Eades
Rustin M Moore
Giselle Hosgood
Changaram S Venugopal
Publication Details: American journal of veterinary research, Vol.66(7), pp.1202-1208
Identifiers: 991005592654807891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.127 Molecular & Cell Biology - Pharmacology; 1.127.1303 Endothelin
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science