Journal article
Chemosensory receptor genes in the Oriental tobacco budworm Helicoverpa assulta
Insect Molecular Biology, Vol.24(2), pp.253-263
2015
Abstract
The Oriental tobacco budworm (Helicoverpa assulta) is a specialist herbivore moth and its larvae feed on Solanaceous plants. (Z)-9-hexadecenal (Z9-16: Ald) is the major sex pheromone component in H. assulta but the specific pheromone receptor (PR) against Z9-16: Ald has not yet been identified. In the present study, we integrated transcriptomic, bioinformatic and functional characterization approaches to investigate the chemosensory receptor genes of H. assulta. We identified seven potential PRs with 44 olfactory receptors, 18 gustatory receptors and 24 ionotropic receptors, which were further studied by in silico gene expression profile, phylogenetic analysis, reverse transcription PCR and calcium imaging assays. The candidate PR, HassOR13, showed a strong response to the minor sex pheromone component, (Z)-11-hexadecenal, but not the major component, Z9-16: Ald, in calcium imaging assays. This study provides the molecular basis for comparative studies of chemosensory receptors between H. assulta and other Helicoverpa species and will advance our understanding of the evolution and function of Lepidoptera insect chemosensation.
Details
- Title
- Chemosensory receptor genes in the Oriental tobacco budworm Helicoverpa assulta
- Authors/Creators
- W. Xu (Author/Creator) - Ecosystem SciencesA. Papanicolaou (Author/Creator) - Ecosystem SciencesN-Y Liu (Author/Creator) - Nanjing Agricultural UniversityS-L Dong (Author/Creator) - Nanjing Agricultural UniversityA. Anderson (Author/Creator) - Ecosystem Sciences
- Publication Details
- Insect Molecular Biology, Vol.24(2), pp.253-263
- Publisher
- Wiley
- Identifiers
- 991005541496307891
- Copyright
- © 2014 The Royal Entomological Society
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.220 Smell & Taste Science
- 3.220.701 Olfactory Systems
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Entomology
- ESI research areas
- Plant & Animal Science