Journal article
Chlamydia trachomatis 60 kDa cysteine rich outer membrane protein: sequence homology between trachoma and LGV biovars
FEMS Microbiology Letters, Vol.65(3), pp.293-297
1989
Abstract
An 820 bp AccI-PstI fragment of the 60 kDa cysteine rich outer membrane protein (CrP) gene from C. trachomatis serovar L1 was used as a probe to locate the 60 kDa CrP gene of a recent serovar B trachoma isolate (Jali 20/OT). The probe hybridized to a single 1.8 kb SpeI fragment in Southern blot analyses of different restriction endonuclease digests of C. trachomatis serovar B DNA. This fragment was ligated into Lambda Zap II arms and Bluescript SK(-) recombinants, release by infection with the helper phage R408, were used as template for DNA sequence determination. Sequence analysis demonstrated a very high level of homology between the Jali 20/OT 60 kDa CrP and the previously published serovar L1 60 kDa CrP with only 8 out of 507 amino acid substitutions between the two proteins.
Details
- Title
- Chlamydia trachomatis 60 kDa cysteine rich outer membrane protein: sequence homology between trachoma and LGV biovars
- Authors/Creators
- M.W. Watson (Author/Creator)P.R. Lambden (Author/Creator)M.E. Ward (Author/Creator)I.N. Clarke (Author/Creator)
- Publication Details
- FEMS Microbiology Letters, Vol.65(3), pp.293-297
- Publisher
- Blackwell Publishing
- Identifiers
- 991005541868507891
- Copyright
- © 2020 Federation of European Microbiological Societies
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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