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Chromosome doubling in monohaploid and dihaploid potatoes by regeneration from cultured leaf explants
Journal article   Peer reviewed

Chromosome doubling in monohaploid and dihaploid potatoes by regeneration from cultured leaf explants

A. Karp, R. Risiott, M.G.K. Jones and S.W.J. Bright
Plant Cell, Tissue and Organ Culture, Vol.3(4), pp.363-373
1984
DOI: https://doi.org/10.1007/bf00043089
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Abstract

Plants were regenerated from cultured excised leaf segments of monohaploid (2n=x=12) and diphaloid (2n=2x=24) potato (Solanum tuberosum L.) and a sample has been studied cytologically. In the case of monohaploids, a single leaf regeneration cycle resulted in almost total recovery of doubled monohaploid plants (2n=2x=24), whilst 50% of the plants regenerated from doubled monohaploid leaves had doubled again to the doubled double monohaploid (or homozygous tetraploid, 2n=4x=48) level. Regeneration from dihaploid leaf pieces also gave a good proportion (60%) of doubled genotypes. Very few mixoploids and very few aneuploids were found. These results, together with the general applicability of the method to a large number of potato cultivars, suggest that it can be used as a simple and reliable method of obtaining homozygous tetraploid potatoes.

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