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Chromosome variation in dividing protoplasts and cell suspensions of wheat
Journal article   Peer reviewed

Chromosome variation in dividing protoplasts and cell suspensions of wheat

A. Karp, Q.S. Wu, S.H. Steele and M.G.K. Jones
Theoretical and Applied Genetics, Vol.74(1), pp.140-146
1987
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Abstract

The cytology of bread wheat (Triticum aestivum) suspension lines, recycled lines (selected for high division frequency) and their dividing protoplasts, have been examined. Extensive numerical and structural chromosome variation was present in all the lines. The most frequently observed chromosome numbers were around 2n=32, indicating that considerable chromosome loss from the normal wheat complement (2n=6x=42) had occurred during selection of the lines. Chromosome aberrations also indicated loss of chromosome arms and chromosome segments. The implications of this variation for studies on transformation and for the potential regeneration of whole plants from protoplasts of bread wheat are discussed.

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