Journal article
Chronic Intra-uterine ureaplasma parvum infection induces injury of the enteric nervous system in ovine fetuses
Frontiers in Immunology, Vol.11, Art. 189
2020
Abstract
Background: Chorioamnionitis, inflammation of the fetal membranes during pregnancy, is often caused by intra-amniotic (IA) infection with single or multiple microbes. Chorioamnionitis can be either acute or chronic and is associated with adverse postnatal outcomes of the intestine, including necrotizing enterocolitis (NEC). Neonates with NEC have structural and functional damage to the intestinal mucosa and the enteric nervous system (ENS), with loss of enteric neurons and glial cells. Yet, the impact of acute, chronic, or repetitive antenatal inflammatory stimuli on the development of the intestinal mucosa and ENS has not been studied. The aim of this study was therefore to investigate the effect of acute, chronic, and repetitive microbial exposure on the intestinal mucosa, submucosa and ENS in premature lambs.
Materials and Methods: A sheep model of pregnancy was used in which the ileal mucosa, submucosa, and ENS were assessed following IA exposure to lipopolysaccharide (LPS) for 2 or 7 days (acute), Ureaplasma parvum (UP) for 42 days (chronic), or repetitive microbial exposure (42 days UP with 2 or 7 days LPS).
Results: IA LPS exposure for 7 days or IA UP exposure for 42 days caused intestinal injury and inflammation in the mucosal and submucosal layers of the gut. Repetitive microbial exposure did not further aggravate injury of the terminal ileum. Chronic IA UP exposure caused significant structural ENS alterations characterized by loss of PGP9.5 and S100β immunoreactivity, whereas these changes were not found after re-exposure of chronic UP-exposed fetuses to LPS for 2 or 7 days.
Conclusion: The in utero loss of PGP9.5 and S100β immunoreactivity following chronic UP exposure corresponds with intestinal changes in neonates with NEC and may therefore form a novel mechanistic explanation for the association of chorioamnionitis and NEC.
Details
- Title
- Chronic Intra-uterine ureaplasma parvum infection induces injury of the enteric nervous system in ovine fetuses
- Authors/Creators
- C. Heymans (Author/Creator) - GROW - School for Oncology and Developmental BiologyI.H. de Lange (Author/Creator) - GROW - School for Oncology and Developmental BiologyM.C. Hütten (Author/Creator) - GROW - School for Oncology and Developmental BiologyK. Lenaerts (Author/Creator) - GROW - School for Oncology and Developmental BiologyN.J.E. de Ruijter (Author/Creator) - GROW - School for Oncology and Developmental BiologyL.C.G.A. Kessels (Author/Creator)G. Rademakers (Author/Creator) - PathologieV. Melotte (Author/Creator) - PathologieW. Boesmans (Author/Creator) - Hasselt UniversityM. Saito (Author/Creator) - Tohoku UniversityH. Usuda (Author/Creator) - Tohoku UniversityS.J. Stock (Author/Creator) - University of EdinburghO.B. Spiller (Author/Creator) - Cardiff UniversityM.S. Payne (Author/Creator) - The University of Western AustraliaB.W. Kramer (Author/Creator) - Nederlandse Vereniging voor KindergeneeskundeJ.P. Newnham (Author/Creator) - The University of Western AustraliaA.H. Jobe (Author/Creator) - Cincinnati Children's Hospital Medical CenterM.W. Kemp (Author/Creator) - Murdoch UniversityW.G. van Gemert (Author/Creator) - RWTH Aachen UniversityT.G.A.M. Wolfs (Author/Creator) - University College Maastricht
- Publication Details
- Frontiers in Immunology, Vol.11, Art. 189
- Publisher
- Frontiers Media
- Identifiers
- 991005541171407891
- Copyright
- © 2020 The Authors
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
66 File views/ downloads
78 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.195 Neuroendocrine & Intestinal Disorders
- 1.195.1341 Enteric Nervous System
- Web Of Science research areas
- Immunology
- ESI research areas
- Immunology