Journal article
Class-specific antibody responses in cattle following experimental challenge with sporocysts or merozoites of Sarcocystis cruzi
Veterinary Parasitology, Vol.72(2), pp.121-127
1997
Abstract
An ELISA using antigen produced from merozoites of Sarcocystis cruzi was developed to monitor specific IgM and IgG antibody, following challenge of cattle with either merozoites or sporocysts of S. cruzi. This assay was compared with an ELISA using antigen produced from the cystozoite stage of the parasite. Both ELISAs were able to detect significant increases in levels of circulating IgM and IgG antibodies against Sarcocystis in all challenged cows; however, the magnitude of the titres was greater in the ELISA which used the antigen derived from the merozoites. This immunoassay also detected increases in the levels of IgG earlier than did the assay using antigen derived from cystozoites of S. cruzi. Since this rise coincided with the presence of clinical signs, and was persistent for several weeks, the IgG-ELISA using antigen derived from merozoites appears to be suitable for the diagnosis of acute sarcocystiosis in cattle. Furthermore, since significant increases in the levels of circulating IgM and IgG antibodies against Sarcocystis were detected in the cows infected with merozoites of S. cruzi, it is evident that merozoites of S. cruzi cultured in vitro maintain their capability to replicate in the natural intermediate host.
Details
- Title
- Class-specific antibody responses in cattle following experimental challenge with sporocysts or merozoites of Sarcocystis cruzi
- Authors/Creators
- G. Savini (Author/Creator) - Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleI.D. Robertson (Author/Creator)J.D. Dunsmore (Author/Creator) - Murdoch University
- Publication Details
- Veterinary Parasitology, Vol.72(2), pp.121-127
- Publisher
- Elsevier BV
- Identifiers
- 991005541136207891
- Copyright
- © 1997 Published by Elsevier B.V.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
28 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.217 Parasitology - Malaria, Toxoplasmosis & Coccidiosis
- 1.217.1038 Toxoplasma Gondii
- Web Of Science research areas
- Parasitology
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science