Clinical prediction of wound re-epithelisation outcomes in non-severe burn injury using the plasma lipidome

Monique J. Ryan; Edward Raby; Reika Masuda; Samantha Lodge; Philipp Nitschke; Garth L. Maker; Julien Wist; Mark W. Fear; Elaine Holmes; Jeremy K. Nicholson; Nicola Gray; Luke Whiley; Fiona M. Wood

doi:10.1016/j.burns.2024.10.003

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Clinical prediction of wound re-epithelisation outcomes in non-severe burn injury using the plasma lipidome

Journal article

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Clinical prediction of wound re-epithelisation outcomes in non-severe burn injury using the plasma lipidome

Monique J. Ryan, Edward Raby, Reika Masuda, Samantha Lodge, Philipp Nitschke, Garth L. Maker, Julien Wist, Mark W. Fear, Elaine Holmes, Jeremy K. Nicholson, …

Burns, Vol.51(1), 107282

2024

DOI: https://doi.org/10.1016/j.burns.2024.10.003

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Abstract

Burn injury

Early prediction

Lipidomics

Metabolic phenotyping

Patient stratification

Wound healing

Whilst wound repair in severe burns has received substantial research attention, non-severe burns (<20% total body surface area) remain relatively understudied, despite causing considerable physiological impact and constituting most of the hospital admissions for burns. Early prediction of healing outcomes would decrease financial and patient burden, and aid in preventing long-term complications from poor wound healing. Lipids have been implicated in inflammation and tissue repair and may play essential roles in burn wound healing. In this study, plasma samples were collected from 20 non-severe burn patients over 6 weeks from admission, including surgery, and analysed by liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance spectroscopy to detect 850 lipids and 112 lipoproteins. Orthogonal projections to latent structures-discriminant analysis was performed to identify changes associated with re-epithelialisation and delayed re-epithelisation. We demonstrated that the lipid and lipoprotein profiles at admission could predict re-epithelisation outcomes at 2 weeks post-surgery, and that these discriminatory profiles were maintained up to 6 weeks post-burn. Inflammatory markers GlycB and C-reactive protein indicated divergent systemic responses to the burn injury at admission. Triacylglycerols, diacylglycerols and low-density lipoprotein subfractions were associated with delayed wound closure (p-value <0.02, Cliff’s delta >0.7), whilst high-density lipoprotein subfractions, phosphatidylinositols, phosphatidylcholines, and phosphatidylserines were associated with re-epithelisation at 2 weeks post-surgery (p-value <0.01, Cliff’s delta <-0.7). Further model validation will potentially lead to personalised intervention strategies to reduce the risk of chronic complications post-burn injury. Abbreviations AUROCArea under receiver operating characteristicsCABINRandomised placebo-controlled trial of Celecoxib for Acute Burn Inflammation and FeverCRPC-reactive proteinCVCross-validatedDIREDiffusion and relaxation editingDGDiacylglycerolDREDelayed wound re-epithelisationFAFatty acylHDLHigh-density lipoproteinIDLIntermediate-density lipoproteinIPAIsopropyl alcoholIQRInter-quartile rangeLC-QQQ-MSLiquid chromatography-tandem mass spectrometryLDCHLow-density lipoprotein cholesterolLDHDLow-density lipoprotein / high-density lipoprotein ratioLDLLow-density lipoproteinLDPLLow-density lipoprotein phospholipidsLPILysophosphatidylinositolNMRNuclear magnetic resonanceOPLS-DAOrthogonal projections to latent structures-discriminant analysisPCPhosphotidylcholinePCAPrincipal component analysisPGPhosphatidylglycerolPIPhosphatidylinositolPLSPartial least squaresPOSASPatient and observer scar assessment scalePSPhosphotidylserineQCQuality controlQTRAPQuadrupole ion trapREWound re-epithelisationSDStandard deviationTBSATotal body surface areaTGTriacylglyerolVLDLVery low-density lipoprotein

Details

Title: Clinical prediction of wound re-epithelisation outcomes in non-severe burn injury using the plasma lipidome
Authors/Creators: Monique J. Ryan - Murdoch University
Edward Raby - Burns Service of Western Australia, WA Department of Health, Murdoch, WA 6150, Australia
Reika Masuda - Australian National Phenome Centre, Health Futures Institute, Harry Perkins Institute, Murdoch University, 5 Robin Warren Drive, Perth, WA 6150, Australia
Samantha Lodge - Australian National Phenome Centre, Health Futures Institute, Harry Perkins Institute, Murdoch University, 5 Robin Warren Drive, Perth, WA 6150, Australia
Philipp Nitschke - Murdoch University
Garth L. Maker - Murdoch University
Julien Wist - Murdoch University
Mark W. Fear - Burn Injury Research Unit, School of Biomedical Sciences, University of Western Australia, Perth, WA 6009, Australia
Elaine Holmes - Murdoch University, Centre for Computational and Systems Medicine
Jeremy K. Nicholson - Murdoch University
Nicola Gray - Murdoch University
Luke Whiley - Australian National Phenome Centre, Health Futures Institute, Harry Perkins Institute, Murdoch University, 5 Robin Warren Drive, Perth, WA 6150, Australia
Fiona M. Wood - Burns Service of Western Australia, WA Department of Health, Murdoch, WA 6150, Australia
Publication Details: Burns, Vol.51(1), 107282
Publisher: Elsevier Ltd
Identifiers: 991005710341207891
Murdoch Affiliation: Australian National Phenome Centre; School of Medical, Molecular and Forensic Sciences; Health Futures Institute; Centre for Computational and Systems Medicine
Language: English
Resource Type: Journal article

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