Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?

S. Siripattanapipong; S. Leelayoova; M. Mungthin; R.C.A. Thompson; P. Boontanom; W. Saksirisampant; P. Tan-ariya

doi:10.1186/1471-2180-11-206

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Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?

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Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?

S. Siripattanapipong, S. Leelayoova, M. Mungthin, R.C.A. Thompson, P. Boontanom, W. Saksirisampant and P. Tan-ariya

BMC Microbiology, Vol.11(1)

2011

DOI: https://doi.org/10.1186/1471-2180-11-206

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Abstract

Background: The glutamate dehydrogenase gene (gdh) is one of the most popular and useful genetic markers for the genotypic analysis of Giardia duodenalis (syn. G. lamblia, G. intestinalis), the protozoan that widely causes enteric disease in humans. To determine the distribution of genotypes of G. duodenalis in Thai populations and to investigate the extent of sequence variation at this locus, 42 fecal samples were collected from 3 regions of Thailand i.e., Central, Northern, and Eastern regions. All specimens were analyzed using PCR-based genotyping and recombinant subcloning methods. Results: The results showed that the prevalence of assemblages A and B among these populations was approximately equal, 20 (47.6%) and 22 (52.4%), respectively. Sequence analysis revealed that the nucleotide diversity of assemblage B was significantly greater than that in assemblage A. Among all assemblage B positive specimens, the allelic sequence divergence within isolates was detected. Nine isolates showed mixed alleles, ranged from three to nine distinct alleles per isolate. Statistical analysis demonstrated the occurrence of genetic recombination within subassemblages BIII and BIV was likely. Conclusion: This study supports increasing evidence that G. duodenalis has the potential for genetic exchange.

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Title: Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?
Authors/Creators: S. Siripattanapipong (Author/Creator) - Mahidol University
S. Leelayoova (Author/Creator) - Phramongkutklao College of Medicine
M. Mungthin (Author/Creator) - Phramongkutklao College of Medicine
R.C.A. Thompson (Author/Creator) - Murdoch University
P. Boontanom (Author/Creator) - Phramongkutklao College of Medicine
W. Saksirisampant (Author/Creator) - Chulalongkorn University
P. Tan-ariya (Author/Creator) - Mahidol University
Publication Details: BMC Microbiology, Vol.11(1)
Publisher: BioMed Central
Identifiers: 991005541795607891
Copyright: © 2011 Siripattanapipong et al
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article
Note: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Microbiology
ESI research areas: Microbiology